See main article: Swimming at the 2010 South American Games. The Women's 100m backstroke event at the 2010 South American Games was held on March 29, with the heats at 10:35 and the Final at 18:10.
| width=200 bgcolor=gold | Gold | width=200 bgcolor=silver | Silver | width=200 bgcolor=CC9966 | Bronze |
| Fabíola Molina | Carolina Colorado Henao | Jeserik Pinto |
| Standing records prior to the 2010 South American Games | |||||
|---|---|---|---|---|---|
| World record | 58.12 | Rome, Italy | 28 July 2009 | ||
| Competition Record | 1:04.39 | Belém, Brazil | 2002 | ||
| South American record | 1:00.07 | Rome, Italy | 1 August 2009 | ||
| Rank | Heat | Lane | width=250 | Athlete ! | width=100 | Result ! | Notes |
|---|---|---|---|---|---|---|---|
| 1 | 2 | 4 | 1:03.17 | Q CR | |||
| 2 | 3 | 4 | 1:04.27 | Q | |||
| 3 | 1 | 4 | 1:05.14 | Q | |||
| 4 | 1 | 5 | 1:05.28 | Q | |||
| 5 | 3 | 3 | 1:05.31 | Q | |||
| 6 | 2 | 6 | 1:06.00 | Q | |||
| 7 | 2 | 5 | 1:06.89 | Q | |||
| 7 | 1 | 6 | 1:07.12 | Q | |||
| 9 | 1 | 3 | 1:07.13 | ||||
| 10 | 1 | 2 | 1:07.63 | ||||
| 11 | 3 | 5 | 1:07.76 | ||||
| 12 | 2 | 2 | 1:07.95 | ||||
| 13 | 2 | 3 | 1:08.78 | ||||
| 14 | 3 | 2 | 1:09.45 | ||||
| 15 | 3 | 1 | 1:10.21 | ||||
| 16 | 3 | 7 | 1:11.17 | ||||
| 17 | 1 | 7 | 1:12.89 | ||||
| 18 | 2 | 7 | 1:13.19 | ||||
| 2 | 1 | DNS | |||||
| 3 | 6 | DSQ | |||||
| Rank | Lane | width=250 | Athlete ! | width=100 | Result ! | Notes |
|---|---|---|---|---|---|---|
| 5 | 1:01.65 | CR | ||||
| 4 | 1:02.77 | |||||
| 6 | 1:04.21 | |||||
| 4 | 3 | 1:04.73 | ||||
| 5 | 2 | 1:05.07 | ||||
| 6 | 7 | 1:05.31 | ||||
| 7 | 8 | 1:06.83 | ||||
| 8 | 1 | 1:07.48 | ||||