The 4 × 100 metres freestyle relay was at Melbourne Sports and Aquatic Centre on 16 March. There was only one heat.
| Rank | Lane | Names | Time | Notes | ||
|---|---|---|---|---|---|---|
| 4 | Roland Schoeman (48.65) Lyndon Ferns (48.43) Gerhard Zandberg (49.44) Ryk Neethling (48.45) | 3:14.97 | GR | |||
| 3 | Michael Klim (49.28) Eamon Sullivan (48.21) Brett Hawke (49.49) Ashley Callus (48.56) | 3:15.54 | ||||
| 6 | Yannick Lupien (49.65) Matthew Rose (48.85) Colin Russell (49.11) Brent Hayden (48.13) | 3:15.74 | ||||
| 4 | 5 | Simon Burnett (48.79) Ross Davenport (48.61) Christopher Cozens (49.89) Anthony Howard (52.27) | 3:19.56 | |||
| 5 | 2 | Craig Houston (51.21) Todd Cooper (50.31) Robert Renwick (50.21) David Carry (49.92) | 3:21.65 | |||
| 6 | 1 | Zhirong Tay (51.98) Lee Yu Tan (52.33) Shirong Su (52.54) Mingzhe Cheah (54.34) | 3:31.19 | |||
| 7 | 7 | Alexis Militis (52.96) Daniel Halksworth (54.75) Liam du Feu (53.39) Simon le Couilliard (53.34) | 3:34.44 | |||
| 8 | 8 | Ben Lowndes (55.11) Jeremy Osborne (swimmer) (53.84) Ian Hubert (54.06) Ian Powell (swimmer) (54.06) | 3:37.07 |