Sulforhodamine B Explained

Sulforhodamine B or Kiton Red 620 (C₂₇H₃₀N₂O₇S₂) is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells. This red solid dye is very water-soluble.^[1]

Spectroscopy

The dye has maximal absorbance at 565 nm light and maximal fluorescence emission at 586 nm light. It does not exhibit pH-dependent absorption or fluorescence over the range of 3 to 10.^[2]

Applications

Sulforhodamine B is often used as a membrane-impermeable polar tracer^[3] or used for cell density determination via determination of cellular proteins (cytotoxicity assay).^[4]

Notes and References

1. Web site: Sulforhodamine B sodium salt (CAS 3520-42-1) . Santa Cruz Biotechnology .
2. Coppeta . J. . Rogers . C. . Dual Emission Laser Induced Fluorescence for Direct Planar Scalar Behavior Measurements . Experiments in Fluids . 1998 . 25 . 1–15 . 10.1007/s003480050202 . 37649159 .
3. Viricel W . Mbarek A . Leblond J . Switchable Lipids: Conformational Change for Fast pH-Triggered Cytoplasmic Delivery . Angewandte Chemie International Edition . 2015 . 54 . 43 . 12743–12747 . 10.1002/anie.201504661 . 26189870. 24175578 .
4. Vichai V . Kirtikara K . Sulforhodamine B colorimetric assay for cytotoxicity screening . Nature Protocols . 2006 . 1 . 3 . 1112–1116 . 10.1038/nprot.2006.179. 17406391 . 1245354 .