| Subprefecture of Vila Maria-Vila Guilherme | |
| Settlement Type: | Subprefecture |
| Image Map1: | SaoPaulo Municip SaoPaulo.svg |
| Map Caption1: | Location of municipality of São Paulo within the State of São Paulo |
| Subdivision Type: | Country |
| Subdivision Name: |  Brazil |
| Subdivision Type1: | Region |
| Subdivision Name1: | Southeast |
| Subdivision Type2: | State |
| Subdivision Name2: | São Paulo |
| Subdivision Type3: | Municipality |
| Subdivision Name3: | São Paulo |
| Subdivision Type4: | Administrative Zone |
| Subdivision Name4: | Northeast |
| Parts Type: | Districts |
| Parts Style: | para |
| P1: | Vila Maria |
| P2: | Vila Guilherme |
| P3: | Vila Medeiros |
| Government Type: | Subprefecture |
| Leader Title: | Subprefect |
| Leader Name: | Antônio de Pádua Perosa |
| Area Total Km2: | 26.87 |
| Population Total: | 287,866 |
| Population As Of: | 2008 |
The Subprefecture of Vila Maria-Vila Guilherme is one of 32 subprefectures of the city of São Paulo, Brazil. It comprises three districts: Vila Maria, Vila Guilherme, and Vila Medeiros.[1]
This is the subprefecture where are the roads for Rio de Janeiro, Minas Gerais and the São Paulo–Guarulhos International Airport (in the neighbouring municipality of Guarulhos), thus forming an important logistic hub of the city, hosting many transport and storage companies.