Structure-Based Assignment (SBA) is a technique to accelerate the resonance assignment which is a key bottleneck of NMR (Nuclear magnetic resonance) structural biology.[1] A homologous (similar) protein is used as a template to the target protein in SBA. This template protein provides prior structural information about the target protein and leads to faster resonance assignment . By analogy, in X-ray Crystallography, the molecular replacement technique allows solution of the crystallographic phase problem when a homologous structural model isknown, thereby facilitating rapid structure determination.[2] Some of the SBA algorithmsare CAP which is an RNA assignment algorithm which performs an exhaustive search overall permutations,[3] MARS which is a program for robust automatic backbone assignment [4] and Nuclear Vector Replacement (NVR) which is a molecular replacement like approach for SBA of resonances andsparse Nuclear Overhauser Effect (NOE)'s.[5] [6] [7]