Streptomyces cattleya explained

Streptomyces cattleya is a Gram-positive bacterium which makes cephamycin,^[1] penicillin and thienamycin.^{[2] [3]} The bacterium expresses a fluorinase enzyme,^[4] and the organism has been used to understand the biosynthesis of fluoroacetate and the antibacterial 4-fluoro-L-threonine.^{[5] [6]} The γ-Glu-βes pathway to biosynthesis of non-traditional amino acids β-ethynylserine (βes) and L-propargylglycine (Pra) was first characterized in this species.^[7]

The genome, which was sequenced in 2011, contains one chromosome with 6,283,062 base pairs and one megaplasmid with 1,809,491 bp, with an overall guanine-cytosine content of 73%.^[8]

Notes and References

1. Bodner. MJ. Li, R . Phelan, RM . Freeman, MF . Moshos, KA . Lloyd, EP . Townsend, CA . Definition of the common and divergent steps in carbapenem β-lactam antibiotic biosynthesis.. ChemBioChem. Sep 19, 2011. 12. 14. 2159–65. 21913298 . 10.1002/cbic.201100366 . 3281309.
2. Kahan. JS . Kahan, FM . Goegelman, R . Currie, SA . Jackson, M . Stapley, EO . Miller, TW . Miller, AK . Hendlin, D . Mochales, S . Hernandez, S . Woodruff, HB . Birnbaum, J. Thienamycin, a new beta-lactam antibiotic. I. Discovery, taxonomy, isolation and physical properties.. The Journal of Antibiotics. Jan 1979. 32. 1. 1–12. 761989. 10.7164/antibiotics.32.1. free .
3. Kahan JS, Kahan FM, Goegelman R, etal . Thienamycin, a new beta-lactam antibiotic. I. Discovery, taxonomy, isolation and physical properties . J. Antibiot. . 32 . 1 . 1–12 . 1979 . 761989 . 10.7164/antibiotics.32.1. free .
4. Biochemistry: Biosynthesis of an organofluorine molecule. Nature. 279. 416. 6878. 10.1038/416279a. David. O'Hagan. Christoph. Schaffrath. Steven L.. Cobb. John T. G.. Hamilton. Cormac D.. Murphy. 11907567. March 2002. 2002Natur.416..279O . 4415511. free.
5. Zhao. C. Li, P . Deng, Z . Ou, HY . McGlinchey, RP . O'Hagan, D . Insights into fluorometabolite biosynthesis in Streptomyces cattleya DSM46488 through genome sequence and knockout mutants.. Bioorganic Chemistry. Oct 2012. 44. 1–7. 22858315 . 10.1016/j.bioorg.2012.06.002.
6. Reid. KA. Hamilton, JT . Bowden, RD . O'Hagan, D . Dasaradhi, L . Amin, MR . Harper, DB . Biosynthesis of fluorinated secondary metabolites by Streptomyces cattleya.. Microbiology. Jun 1995. 141. 1385–93. 7670640 . 6 . 10.1099/13500872-141-6-1385.
7. Kuehnel. Karin. May 2019. A bacterial treasure trove. Nature Methods. Paper. 16. 5. 360. 10.1038/s41592-019-0411-1. 31040424. free.
8. Barbe. V. Bouzon, M . Mangenot, S . Badet, B . Poulain, J . Segurens, B . Vallenet, D . Marlière, P . Weissenbach, J . Complete genome sequence of Streptomyces cattleya NRRL 8057, a producer of antibiotics and fluorometabolites.. Journal of Bacteriology. Sep 2011. 193. 18. 5055–6. 21868806 . 10.1128/JB.05583-11 . 3165681.