Stiriinae is a subfamily of owlet moths in the family Noctuidae. There are more than 20 genera and 130 described species in Stiriinae.
The current classification of Stiriinae is based on phylogenetic research published in 2019 in which the tribe Stiriini was determined to be polyphyletic, with much of its diversity spread among three subfamilies, and a follow-up study in 2021 that found (at the time) non-Stiriinae genera grouped within Stiriinae in phylogenetic analyses. As a result of these studies, the tribe Stiriini was elevated to subfamily status as Stiriiinae, Grotellina, a subtribe of Stiriini before the 2019 study, was elevated to subfamily status as Grotellinae, and various other genera that were found to group in the closely related subfamily Metoponiinae were transferred into it. Stiriini and Annaphilini are now tribes of the currently recognized Stiriinae (with no subtribes within either) containing the majority of genera from the former subtribes Stiriina and Annaphilina that had existed prior to the 2019 study.
These 21 genera belong to the subfamily Stiriinae: