| State: | FL |
| Type: | SR |
| Route: | 320 |
| Length Mi: | 5.784 |
| Map Custom: | yes |
| Map Notes: | SR 320 in red, CR 320 in blue |
| Direction A: | West |
| Terminus A: | Manatee Springs State Park |
| Direction B: | East |
| Terminus B: | in Chiefland |
| Counties: | Levy |
| Previous Type: | US |
| Previous Route: | 319 |
| Next Type: | SR |
| Next Route: | 326 |
State Road 320 (SR 320) is an east - west route in Levy County connecting Manatee Springs State Park to U.S. Route 19 (US 19), 98, and US 27 Alternate (US 27 Alt.).
The state-signed portion runs along streets named Manatee Springs Road, Northwest 115th Street, and Northwest 19th Avenue. The road continues east of US 19/98/27 Alt. as County Road 320 along Northeast 120th Street from its intersection with US 19/98/27 Alt. to CR 339 in Newton.