Squalene—-hopanol cyclase explained

Squalene—hopanol cyclase
Ec Number:	4.2.1.129

Squalene—hopanol cyclase (squalene—hopene cyclase) is an enzyme with systematic name hopan-22-ol hydro-lyase.^{[1] [2]} This enzyme catalyses the following chemical reaction

hopan-22-ol

\rightleftharpoons squalene + H₂O

The enzyme produces the cyclization products hopene and hopanol.

Notes and References

1. Hoshino T, Nakano S, Kondo T, Sato T, Miyoshi A . Squalene-hopene cyclase: final deprotonation reaction, conformational analysis for the cyclization of (3R,S)-2,3-oxidosqualene and further evidence for the requirement of an isopropylidene moiety both for initiation of the polycyclization cascade and for the formation of the 5-membered E-ring . Organic & Biomolecular Chemistry . 2 . 10 . 1456–70 . May 2004 . 15136801 . 10.1039/b401172d .
2. Sato T, Kouda M, Hoshino T . Site-directed mutagenesis experiments on the putative deprotonation site of squalene-hopene cyclase from Alicyclobacillus acidocaldarius . Bioscience, Biotechnology, and Biochemistry . 68 . 3 . 728–38 . March 2004 . 15056909 . 10.1271/bbb.68.728 . free .