| Spring Brook | |
| Pushpin Map: | USA New York |
| Pushpin Map Caption: | Location of the mouth of Spring Brook |
| Subdivision Type1: | Country |
| Subdivision Name1: | United States |
| Subdivision Type2: | State |
| Subdivision Name2: | New York |
| Subdivision Type3: | Region |
| Subdivision Name3: | Central New York Region |
| Subdivision Type4: | County |
| Subdivision Name4: | Otsego |
| Source1: | Arnold Lake |
| Source1 Location: | Milford, New York |
| Source1 Coordinates: | 42.6119°N -75.0089°W |
| Source1 Elevation: | 1775feet |
| Mouth: | Goodyear Lake |
| Mouth Location: | Portlandville, New York |
| Mouth Coordinates: | 42.5286°N -74.9722°W[1] |
| Mouth Elevation: | 1138feet |
Spring Brook is a river located in central Otsego County, New York. The creek drains Arnold Lake and flows south before converging with Goodyear Lake, which is an impoundment of the Susquehanna River, by Portlandville, New York.