| Sixteen Mile Creek | |
| Pushpin Map: | Canada Southern Ontario |
| Pushpin Map Caption: | Location of the mouth of Sixteen Mile Creek in southern Ontario |
| Subdivision Type1: | Country |
| Subdivision Name1: | Canada |
| Subdivision Type2: | Province |
| Subdivision Name2: | Ontario |
| Subdivision Type3: | Region |
| Subdivision Name3: | Southern Ontario |
| Subdivision Type4: | Regional Municipality |
| Subdivision Name4: | Niagara |
| Subdivision Type5: | Municipalities |
| Source1: | Field |
| Source1 Location: | West Lincoln |
| Source1 Coordinates: | 43.0614°N -79.5239°W |
| Source1 Elevation: | 182m (597feet) |
| Mouth: | Sixteen Mile Creek Pond at Lake Ontario |
| Mouth Location: | Lincoln |
| Mouth Coordinates: | 43.1594°N -79.3339°W |
| Mouth Elevation: | 74m (243feet) |
| River System: | Great Lakes Basin |
Sixteen Mile Creek is a stream in the municipalities of Lincoln, Pelham and West Lincoln, Regional Municipality of Niagara in Southern Ontario, Canada.[1] It is part of the Great Lakes Basin and flows from the top of the Niagara Escarpment to Sixteen Mile Pond on Lake Ontario. The stream falls within the remit of the Niagara Peninsula Conservation Authority,[2] whose 2012 watershed report card gave the stream watershed grades of D for both water quality and forest cover.