| Sixteen Mile Creek | |
| Pushpin Map: | Canada Southern Ontario |
| Pushpin Map Caption: | Location of the mouth of Sixteen Mile Creek in southern Ontario |
| Subdivision Type1: | Country |
| Subdivision Name1: | Canada |
| Subdivision Type2: | Province |
| Subdivision Name2: | Ontario |
| Subdivision Type3: | Region |
| Subdivision Name3: | Central Ontario |
| Subdivision Type4: | District Municipality |
| Subdivision Name4: | Muskoka |
| Subdivision Type5: | Municipality |
| Subdivision Name5: | Lake of Bays |
| Source1: | Hickory Lake |
| Source1 Coordinates: | 45.4061°N -78.9953°W |
| Source1 Elevation: | 418m (1,371feet) |
| Mouth: | Boyne River |
| Mouth Coordinates: | 45.3364°N -79.0158°W |
| Mouth Elevation: | 320m (1,050feet) |
| River System: | Great Lakes Basin |
Sixteen Mile Creek is a stream in the municipality of Lake of Bays, District Municipality of Muskoka in Central Ontario, Canada.[1] It is part of the Great Lakes Basin and flows from Hickory Lake to its mouth at the Boyne River. The Boyne River flows to the Lake of Bays, then via the Muskoka River, and the Moon River and Musquash River to Georgian Bay on Lake Huron.[2]