Sirius Red Explained

Sirius Red F 3B (Direct Red 80) is an azo dye primarily used in staining methods for collagen and amyloid.^[1] It has the molecular formula C₄₅H₂₆N₁₀Na₆O₂₁S₆.

In histology, sirius red staining is used in various domains of diagnostic to observe fibrosis levels in a lot of cases of inflammation induced by cancer, vascular or metabolic pathologies.^[2]

In bright field microscopy the following can be observed:

• The nuclei in yellow
• The cytoplasm in yellow
• Collagen fibers in red
• Muscular fibers in yellow
• Red blood cells in yellow

See also

• Collagen Hybridizing Peptide, a peptide that stains denatured collagen in tissues

References

1. Certification procedures for sirius red F3B . 2015-08-21 . March 2011 . Biotechnic & Histochemistry . 86 . 3 . 133–9 . Dapson, Richard W. . Fagan, C. . Kiernan, John A. . Wickersham, T.W. . 10.3109/10520295.2011.570277. 21417582 . 32655644 .
2. Web site: Sirius red . Histalim . 2015-09-22.