Sf9 (cells) explained
Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-Sf21-AE), are commonly used in insect cell culture for recombinant protein production using baculovirus. They were originally established from ovarian tissue.[1] They can be grown in the absence of serum, and can be cultured attached or in suspension.[2]
Sf9 Rhabdovirus
It has previously been shown that some Sf9 cell lines harbor a negative sense Rhabdovirus called Spodoptera frugiperda rhabdovirus (SfRV).[3] [4] However, not all tested Sf9 cells appear to be infected with this virus.[5] SfRV appears to be insect-specific and does not appear to infect mammalian cell lines.[6]
References
- Vaughn JL . Goodwin RH . Tompkins GJ . McCawley P . The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae) . In Vitro . 1977 . 13 . 4 . 213–217 . 68913 . 10.1007/BF02615077. 13529129 .
- Book: Invitrogen. Growth and Maintenance of Insect cell lines: User Guide . Cell Lines . Rev. Date - 8 March 2010 . Invitrogen . 14 Mar 2017 . Manual part no. 25-0127, MAN0000030 . 12 May 2017 .
- Ma H, Galvin TA, Glasner DR, Shaheduzzaman S, Khan AS. Identification of a novel rhabdovirus in Spodoptera frugiperda cell lines. . J Virol . 2014 . 88 . 12 . 6576–85 . 24672045 . 10.1128/JVI.00780-14 . 4054387 .
- Parry R, de Malmanche H, Asgari S. Persistent Spodoptera frugiperda rhabdovirus infection in Sf9 cells is not restricted by Wolbachia wMelPop-CLA and wAlbB strains and is targeted by the RNAi machinery. . Virology . 2021 . 563 . 82–87 . 34492433 . 10.1016/j.virol.2021.08.013 . free .
- Hashimoto Y, Macri D, Srivastava I, McPherson C, Felberbaum R, Post P . etal. Complete study demonstrating the absence of rhabdovirus in a distinct Sf9 cell line. . PLOS ONE . 2017 . 12 . 4 . e0175633 . 28423032 . 10.1371/journal.pone.0175633 . 5397025 . 2017PLoSO..1275633H. free.
- Maghodia AB, Jarvis DL. Infectivity of Sf-rhabdovirus variants in insect and mammalian cell lines. . Virology . 2017 . 512 . 234–245 . 29024851 . 10.1016/j.virol.2017.09.025 . 5651996 .
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