| Seymour | |
| Type: | Regional rail |
| Status: | Operational |
| Locale: | Victoria, Australia |
| Operator: | V/Line |
| Ridership: | 1.29 million (2022–23)[1] |
| Start: | Seymour |
| Stops: | 13 |
| End: | Southern Cross |
| Distance: | 101.69km (63.19miles) |
| Journeytime: | 1 hour 32 minutes |
| Line Used: | Tocumwal |
| Class: | Economy |
| Access: | Most services |
| Catering: | No |
| Stock: | [2] |
| El: | 1500 V DC overhead |
| Speed: | 130km/h maximum[3] |
| Owners: | VicTrack |
The Seymour Line is a regional passenger rail service operated by V/Line in Victoria, Australia. It serves passengers between the state capital of Melbourne and the regional city of Seymour, along with a number of smaller communities in between.
It is the fifth most-used regional rail service in Victoria (behind the Traralgon, Bendigo, Ballarat, and Geelong services), carrying 1.29 million passengers in the 2022–23 financial year.
V/Line operates hourly passenger services to Melbourne, with Sprinter and VLocity railcars used on quieter services, as well as locomotive hauled H type carriages during peak times. Beyond Seymour, three daily passenger trains run as the Albury Line and five daily services run as the Shepparton Line.