Serum free light-chain measurement explained

Serum free light-chain measurement
Purpose:Measurement of the serum level of FLCs

Free light chains (FLCs) are immunoglobulin light chains that are found in the serum (blood) in an unbound (free) state. In recent decades, measuring the amount of free light chains (FLCs) in the blood has become a practical clinical test. FLC tests can be used to diagnose and monitor diseases like multiple myeloma and amyloidosis.

Structure

Each immunoglobulin light-chain molecule contains approximately 220 amino acids in a single polypeptide chain that is folded to form constant and variable region domains.Each domain comprises two β-pleated sheets. The sheets are linked by a disulfide bridge and together form a roughly barrel-shaped structure known as a β-barrel.The variable (V) domain of light chains has a high degree of structural diversity, particularly the antigen-binding region. In addition, the first 23 amino acids of the 1st variable domain framework region have a number of variations known as subgroups. Four kappa (Vκ1–Vκ4) and six lambda subgroups (Vλ1–Vλ6) can be identified.[1] The subgroup structures of FLCs influence their ability to polymerize (combine) and form proteins like amyloid fibrils. For example, the Vλ6 subgroup of FLCs is associated with a type of amyloidosis called AL amyloidosis, while the Vκ1 and Vκ4 subgroups are associated with a different type of amyloidosis called light-chain deposition disease.[2]

Synthesis

Kappa light-chain molecules are constructed from approximately 40 functional Vκ gene segments (chromosome 2), five Jκ gene segments and a single Cκ gene. Lambda molecules (chromosome 22) are constructed from about 30 Vλ gene segments and four pairs of functional Jλ gene segments and a Cλ gene.[3]

Light chains are incorporated into immunoglobulin molecules during B-cell development and are expressed initially on the surface of pre B-cells. Production of light chains occurs throughout the rest of B-cell development and in plasma cells, where secretion is highest.

Production

The production of free immunoglobulin light chains in normal individuals is approximately 500 mg/day from bone marrow and lymph node cells.[1] [4] The production of immunoglobulin light chains is about 40% greater than the production of immunoglobulin heavy chains. This may simply be to allow for the proper structure of the intact immunoglobulin molecules, but it is also possible that free light chains have an immunological function.[5] There are approximately twice as many kappa-producing plasma cells as lambda plasma cells. Kappa free-light chains are normally monomeric, while lambda free-light chains tend to be dimeric, joined by disulphide bonds. Polymeric forms of both types of free light chain can also occur.[6]

Metabolism

In normal individuals, free light chains are rapidly cleared from the blood and catabolised by the kidneys. Monomeric free light chains are cleared in 2–4 hours, and dimeric light chains in 3–6 hours.[7] Removal may be prolonged to 2–3 days in people with complete renal failure.[1] [4] [8] Human kidneys are composed of approximately half a million nephrons. Each nephron contains a glomerulus with basement membrane pores that allow filtration of immunoglobulin light chains and other small molecules from the blood into the proximal tubule of the nephron.

Filtered molecules are either excreted in the urine or may be specifically re-absorbed. Protein molecules that pass through the glomerular pores are either absorbed unchanged (such as albumin), degraded in the proximal tubular cells and absorbed (such as free light chains), or excreted as fragments.[9] This re-absorption is mediated by a receptor complex (megalin/cubulin) and prevents the loss of large amounts of protein into the urine. It is very efficient and can process 10–30 g of low-molecular-weight proteins per day, so under normal conditions no light chains pass beyond the proximal tubules.[10] [11] [12]

If immunoglobulin light chains are produced in sufficient amounts to overwhelm the proximal tubules' absorption mechanisms (usually due to the presence of a plasma cell tumour) the light chains enter the distal tubules and can appear in the urine (Bence Jones protein). The passage of large amounts of immunoglobulin light chains through the kidneys may cause inflammation or blockage of the kidney tubules.

The distal tubules of the kidneys secrete large amounts of uromucoid (Tamm–Horsfall protein). This is the dominant protein in normal urine and is thought to be important in preventing ascending urinary infections. It is a relatively small glycoprotein (80 kDa) that aggregates into polymers of 20–30 molecules. It contains a short amino-acid sequence that can specifically bind to some free light chains.[13] Together they can form an insoluble precipitate which blocks the distal part of the nephrons. This is termed "cast nephropathy" or "myeloma kidney" and is typically found in patients with multiple myeloma.[14] [15] This can block the flow of urine causing the death of the respective nephrons. Rising concentrations of light chains are filtered by the remaining nephrons leading to a cycle of accelerating renal damage with rising concentrations of free light chains in the blood.[16] At the same time, the amount of free light chains entering the urine will be decreased and will be zero if the patient stops producing urine (anuria). Conversely, urine concentrations of free light chains could increase if renal function improved in a multiple myeloma patient receiving treatment. This could account for the poor correlation frequently seen when urine and serum free light-chain concentrations are compared.[17] [18] [19] [20]

The 500 mg of FLCs produced per day by the normal lymphoid system, however, flows through the glomeruli and is completely processed by the proximal tubules. If the proximal tubules of the nephrons are damaged or stressed (such as in hard exercise), filtered FLCs may not be completely metabolised and small amounts may then appear in the urine.[9]

Clinical use

Serum free light-chain assays have been used in a number of published studies which have indicated superiority over the urine tests, particularly for patients producing low levels of monoclonal free light chains, as seen in nonsecretory multiple myeloma[21] [22] [23] and AL amyloidosis.[23] [24] [25] [26] This is primarily because of the re-absorption of free light chains in the kidneys, creating a threshold of light chain production which must be exceeded before measurable quantities overflow into the urine.[17] [18] [19] While there are a number of publications indicating that serum free light chain analysis is preferable to urine analysis at diagnosis,[27] [28] [29] [30] there is currently no consensus on whether urine tests for monitoring should be replaced.[18] [19] [20] [31]

A series of studies, principally from the Mayo Clinic, have indicated that patients with an abnormal free kappa to free lambda ratio have an increased risk of progression to active myeloma from precursor conditions including monoclonal gammopathy of undetermined significance (MGUS),[32] [33] smouldering myeloma[34] and solitary plasmacytoma of the bone.[35] Abnormal free light chain production has also been reported to be prognostic of a worse outcome in multiple myeloma[36] [37] [38] and chronic lymphocytic leukaemia.[39] An abnormal light-chain ratio has been defined as a kappa to lambda chain ratio of less than 0.26 or more than 1.65.[32]

Guidelines

In 2009, the International Myeloma Working Group published guidelines making recommendations of when serum free light-chain analysis should be used in the management of multiple myeloma.[40]

Diagnosis

The serum free light-chain assay in combination with serum protein electrophoresis and serum immunofixation electrophoresis is sufficient to screen for pathological monoclonal plasmaproliferative disorders other than AL amyloidosis which requires all the serum tests as well as 24 h urine immunofixation electrophoresis.

Monitoring

Serial serum free light-chain measurement should be routinely performed in patients with AL amyloidosis and multiple myeloma patients with oligosecretory disease. It should also be done in all patients who have achieved a complete response to treatment to determine whether they have attained a stringent complete response.

Other guidelines for the use of serum free light chain measurement in the management of AL amyloidosis,[41] plasmacytoma[42] and the comparison of treatment responses in clinical trials[43] have also been published.

Technical and clinical reviews of serum free light-chain measurement have recently been written by Pratt and Jagannath.[44] [45]

External links

Notes and References

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  2. Basnayake . Kolitha . Stringer . Stephanie J. . Hutchison . Colin A. . Cockwell . Paul . 2011-06-02 . The biology of immunoglobulin free light chains and kidney injury . Kidney International . 79 . 12 . 1289–1301 . 10.1038/ki.2011.94 . 0085-2538. free . 21490587 .
  3. Janeway CA, Travers P, Walport M, Slomchik MJ, "Immunobiology; the immune system in health and disease" (2005); Garland Science publishing.
  4. Waldmann TA, Strober W, Mogielnicki RP . The renal handling of low molecular weight proteins: II. Disorders of serum protein catabolism in patients with tubular proteinuria, the nephrotic syndrome, or uremia . The Journal of Clinical Investigation . 51 . 8 . 2162–74 . August 1972 . 5054468 . 292373 . 10.1172/JCI107023. Strober . Mogielnicki .
  5. Redegeld FA, Nijkamp FP . Immunoglobulin free light chains and mast cells: pivotal role in T-cell-mediated immune reactions? . Trends in Immunology . 24 . 4 . 181–5 . April 2003 . 12697449 . 10.1016/S1471-4906(03)00059-0. Nijkamp .
  6. Sölling K . Polymeric forms of free light chains in serum from normal individuals and from patients with renal diseases . Scandinavian Journal of Clinical and Laboratory Investigation . 36 . 5 . 447–52 . September 1976 . 824709 . 10.3109/00365517609054462.
  7. Meittinen . TA . Effect of imparied hepatic and renal function in [131I] Bence Jones Protein catabolism in human subjects . Clinica Chimica Acta . 1967 . 18 . 395-407. 10.1016/0009-8981(67)90036-8 .
  8. 10.1016/0009-8981(67)90036-8 . Effect of impaired hepatic and renal function on [131]bence jones protein catabolism in human subjects . 1967 . Miettinen, T . Clinica Chimica Acta . 18 . 395 . Kekki . M. 3.
  9. 11979334 . 10.1053/ajkd.2002.32764 . 39 . 5 . Renal handling of albumin: a critical review of basic concepts and perspective . May 2002 . Am. J. Kidney Dis. . 899–919 . Russo LM, Bakris GL, Comper WD. Bakris . Comper .
  10. Abraham GN, Waterhouse C . Evidence for defective immunoglobulin metabolism in severe renal insufficiency . The American Journal of the Medical Sciences . 268 . 4 . 227–33 . October 1974 . 4217565 . 10.1097/00000441-197410000-00003. Waterhouse . 26350666 .
  11. Wochner RD, Strober W, Waldmann TA . The Role of the Kidney in the Catabolism of Bence Jones Proteins and Immunoglobulin Fragments . The Journal of Experimental Medicine . 126 . 2 . 207–21 . August 1967 . 4165739 . 2138312 . 10.1084/jem.126.2.207. Strober . Waldmann .
  12. Maack T, Johnson V, Kau ST, Figueiredo J, Sigulem D . Renal filtration, transport, and metabolism of low-molecular-weight proteins: a review . Kidney International . 16 . 3 . 251–70 . September 1979 . 393891 . 10.1038/ki.1979.128. Johnson . Kau . Figueiredo . Sigulem . free .
  13. Ying WZ, Sanders PW . Mapping the Binding Domain of Immunoglobulin Light Chains for Tamm-Horsfall Protein . The American Journal of Pathology . 158 . 5 . 1859–66 . 1 May 2001 . 11337384 . 1891942 . 10.1016/S0002-9440(10)64142-9 . Sanders .
  14. Sanders PW, Booker BB, Bishop JB, Cheung HC . Mechanisms of intranephronal proteinaceous cast formation by low molecular weight proteins . The Journal of Clinical Investigation . 85 . 2 . 570–6 . February 1990 . 2298921 . 296460 . 10.1172/JCI114474. Booker . Bishop . Cheung .
  15. Sanders PW, Booker BB . Pathobiology of cast nephropathy from human Bence Jones proteins . The Journal of Clinical Investigation . 89 . 2 . 630–9 . February 1992 . 1737851 . 442896 . 10.1172/JCI115629. Booker .
  16. Book: Merlini G, Pozzi C . Mechanisms of renal damage in plasma cell dyscrasias: an overview . 153 . 66–86 . 2007 . 17075224 . 10.1159/000096761. Contributions to Nephrology. 978-3-8055-8178-3. Pozzi .
  17. Bradwell AR, Carr-Smith HD, Mead GP, Harvey TC, Drayson MT . Serum test for assessment of patients with Bence Jones myeloma . Lancet . 361 . 9356 . 489–91 . February 2003 . 12583950 . 10.1016/S0140-6736(03)12457-9. Carr-Smith . Mead . Harvey . Drayson . 43483748 .
  18. Alyanakian MA, Abbas A, Delarue R, Arnulf B, Aucouturier P . Free immunoglobulin light-chain serum levels in the follow-up of patients with monoclonal gammopathies: correlation with 24-hr urinary light-chain excretion . American Journal of Hematology . 75 . 4 . 246–8 . April 2004 . 15054820 . 10.1002/ajh.20007. Abbas . Delarue . Arnulf . Aucouturier . 1067413 . free .
  19. Nowrousian MR, Brandhorst D, Sammet C . 1290359 . Serum free light chain analysis and urine immunofixation electrophoresis in patients with multiple myeloma . Clinical Cancer Research . 11 . 24 Pt 1 . 8706–14 . December 2005 . 16361557 . 10.1158/1078-0432.CCR-05-0486 . etal .
  20. Dispenzieri A, Zhang L, Katzmann JA . Appraisal of immunoglobulin free light chain as a marker of response . Blood . 111 . 10 . 4908–15 . May 2008 . 18364469. 2964259 . 10.1182/blood-2008-02-138602 . etal .
  21. Drayson M, Tang LX, Drew R, Mead GP, Carr-Smith H, Bradwell AR. Serum free light-chain measurements for identifying and monitoring patients with nonsecretory multiple myeloma. Blood . 97 . 9 . 2900–2 . May 2001. 11313287 . 10.1182/blood.V97.9.2900. Tang. Drew . Mead . Carr-Smith . Bradwell . 8779162.
  22. Shaw GR . Nonsecretory plasma cell myeloma—becoming even more rare with serum free light-chain assay: A brief review . Archives of Pathology & Laboratory Medicine. 130 . 8 . 1212–5 . August 2006 . 16879026. 10.5858/2006-130-1212-NPCMEM.
  23. Katzmann JA, Abraham RS, Dispenzieri A, Lust JA, Kyle RA. Diagnostic performance of quantitative kappa and lambda free light chain assays in clinical practice. Clinical Chemistry . 51 . 5 . 878–81. May 2005 . 15774572 . 10.1373/clinchem.2004.046870. Abraham . Dispenzieri . Lust . Kyle . free .
  24. Lachmann HJ, Gallimore R, Gillmore JD. Outcome in systemic AL amyloidosis in relation to changes in concentration of circulating free immunoglobulin light chains following chemotherapy. British Journal of Haematology . 122 . 1. 78–84 . July 2003 . 12823348. 10.1046/j.1365-2141.2003.04433.x . 23475887. etal .
  25. Abraham . RS . Katzmann . JA . Clark . RJ . Bradwell . AR . Kyle . RA . Gertz . MA. Quantitative analysis of serum free light chains: A new marker for the diagnostic evaluation of primary systemic amyloidosis. American Journal of Clinical Pathology . 119 . 2 . 274–78. February 2003 . 12579999 . 10.1309/LYWM-47K2-L8XY-FFB3. free .
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  27. Hill PG, Forsyth JM, Rai B, Mayne S. Serum free light chains: An alternative to the urine Bence Jones proteins screening test for monoclonal gammopathies. Clinical Chemistry . 52 . 9 . 1743–8 . September 2006 . 16858075 . 10.1373/clinchem.2006.069104. Forsyth . Rai . Mayne . free .
  28. Bakshi NA, Gulbranson R, Garstka D, Bradwell AR, Keren DF. Serum free light chain (FLC) measurement can aid capillary zone electrophoresis in detecting subtle FLC-producing M proteins. American Journal of Clinical Pathology . 124 . 2 . 214–18. August 2005 . 16040291 . 10.1309/XE3U-DARK-W1B9-EMWM. Gulbranson . Garstka . Bradwell . Keren . free .
  29. Abadie JM, Bankson DD. Assessment of serum free light chain assays for plasma cell disorder screening in a Veterans Affairs population. Annals of Clinical and Laboratory Science. 36 . 2 . 157–62 . 2006. 16682511. Bankson .
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  31. Abraham RS, Clark RJ, Bryant SC. Correlation of serum immunoglobulin free light chain quantification with urinary Bence Jones protein in light chain myeloma. Clinical Chemistry . 48 . 4 . 655–57 . 1 April 2002. 10.1093/clinchem/48.4.655. 11901068 . etal . free.
  32. Rajkumar SV, Kyle RA, Therneau TM. Serum free light chain ratio is an independent risk factor for progression in monoclonal gammopathy of undetermined significance. Blood . 106 . 3 . 812–7 . August 2005. 15855274 . 1895159 . 10.1182/blood-2005-03-1038. etal .
  33. Rajkumar SV, Lacy MQ, Kyle RA. Monoclonal gammopathy of undetermined significance and smoldering multiple myeloma. Blood Reviews . 21 . 5 . 255–65. September 2007 . 17367905 . 3904304. 10.1016/j.blre.2007.01.002. Lacy . Kyle .
  34. Dispenzieri A, Kyle RA, Katzmann JA. Immunoglobulin free light chain ratio is an independent risk factor for progression of smoldering (asymptomatic) multiple myeloma. Blood . 111 . 2 . 785–9 . January 2008. 17942755 . 2200851 . 10.1182/blood-2007-08-108357 . etal .
  35. Dingli D, Kyle RA, Rajkumar SV. Immunoglobulin free light chains and solitary plasmacytoma of bone. Blood . 108 . 6 . 1979–83. September 2006 . 16741249 . 1895544. 10.1182/blood-2006-04-015784 . etal .
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  38. van Rhee F, Bolejack V, Hollmig K. High serum-free light chain levels and their rapid reduction in response to therapy define an aggressive multiple myeloma subtype with poor prognosis. Blood . 110 . 3 . 827–32 . August 2007. 17416735 . 1924775 . 10.1182/blood-2007-01-067728. etal .
  39. Pratt G, Harding S, Holder R. Abnormal serum free light chain ratios are associated with poor survival and may reflect biological subgroups in patients with chronic lymphocytic leukaemia. British Journal of Haematology . 144 . 2 . 217–22. January 2009 . 19016722 . 10.1111/j.1365-2141.2008.07456.x. 28394748. etal .
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  45. Jagannath S. Value of serum free light chain testing for the diagnosis and monitoring of monoclonal gammopathies in hematology. Clinical Lymphoma & Myeloma . 7 . 8 . 518–23. September 2007 . 18021469 . 10.3816/CLM.2007.n.036.

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