Sereny test explained

The Sereny test is a test used to test the invasiveness of enteroinvasive Escherichia coli, Shigella species, and Listeria monocytogenes.^{[1] [2] [3]}

It is done by inoculating suspension of bacteria into guinea pig's eye. Severe mucopurulent conjunctivitis and severe keratitis indicates a positive test.^[3]

Notes and References

1. Wood PK, Morris JG, Small PL, etal . Comparison of DNA probes and the Sereny test for identification of invasive Shigella and Escherichia coli strains . J. Clin. Microbiol. . 24 . 3 . 498–500 . September 1986 . 10.1128/jcm.24.3.498-500.1986 . 3531233 . 268950 .
2. Murayama SY, Sakai T, Makino S, Kurata T, Sasakawa C, Yoshikawa M . The use of mice in the Sereny test as a virulence assay of shigellae and enteroinvasive Escherichia coli . Infect. Immun. . 51 . 2 . 696–8 . February 1986 . 10.1128/iai.51.2.696-698.1986 . 3510985 . 262412 .
3. Book: Modern Food Microbiology . 21 April 2018 . Jay JM . 262 . 1995 . Springer . 5 . 978-1-4615-7476-7.