Senescence-associated beta-galactosidase explained
| galactosidase, beta 1 |
| Hgncid: | 4298 |
| Symbol: | GLB1 |
| Altsymbols: | SA-β-Gal |
| Entrezgene: | 2720 |
| Omim: | 611458 |
| Refseq: | NM_000404 |
| Uniprot: | P16278 |
| Ecnumber: | 3.2.1.23 |
| Chromosome: | 3 |
| Arm: | p |
| Band: | 22.3 |
Senescence-associated beta-galactosidase (SA-β-gal or SABG) is a hypothetical hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides only in senescent cells. Senescence-associated beta-galactosidase, along with p16Ink4A, is regarded to be a biomarker of cellular senescence.[1] [2]
Its existence was proposed in 1995 by Dimri et al.[3] following the observation that when beta-galactosidase assays were carried out at pH 6.0, only cells in senescence state develop staining. They proposed a cytochemical assay based on production of a blue-dyed precipitate that results from the cleavage of the chromogenic substrate X-Gal, which stains blue when cleaved by galactosidase. Since then, even more specific quantitative assays were developed for its detection at pH 6.0.[4] [5] [6]
Today this phenomenon is explained by the overexpression and accumulation of the endogenous lysosomal beta-galactosidase specifically in senescent cells.[7] Its expression is not required for senescence.[8] However, it remains as the most widely used biomarker for senescent and aging cells, because it is easy to detect and reliable both in situ and in vitro.
Further reading
- Yang NC, Hu ML . The limitations and validities of senescence associated-beta-galactosidase activity as an aging marker for human foreskin fibroblast Hs68 cells . Exp. Gerontol. . 40 . 10 . 813–9 . October 2005 . 16154306 . 10.1016/j.exger.2005.07.011 . 30500511 .
Notes and References
- Hall BM, Balan V, Gleiberman AS, Strom E, Krasnov P, Virtuoso LP, Rydkina E, Vujcic S, Balan K, Gitlin I, Leonova K, Polinsky A, Chernova OB, Gudkov AV . Aging of mice is associated with p16(Ink4a)- and β-galactosidase-positive macrophage accumulation that can be induced in young mice by senescent cells . . 8 . 7 . 1294–1315 . 2016 . 10.18632/aging.100991 . 4993332 . 27391570.
- 2017. p16(Ink4a) and senescence-associated β-galactosidase can be induced in macrophages as part of a reversible response to physiological stimuli. Aging. Albany NY. 9. 1867–1884. 10.18632/aging.101268. Hall BM, Balan V, Gleiberman AS, Strom E, Krasnov P, Virtuoso LP, Rydkina E, Vujcic S, Balan K, Gitlin II, Leonovo KI, Consiglio CR, Gollnick SO, et al.. 8. 5611982. 28768895.
- Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, Medrano EE, Linskens M, Rubelj I, Pereira-Smith O . A biomarker that identifies senescent human cells in culture and in aging skin in vivo . Proc. Natl. Acad. Sci. U.S.A. . 92 . 20 . 9363–7 . September 1995 . 7568133 . 40985 . 10.1073/pnas.92.20.9363. 1995PNAS...92.9363D . free .
- Bassaneze V, Miyakawa AA, Krieger JE . A quantitative chemiluminescent method for studying replicative and stress-induced premature senescence in cell cultures . Anal. Biochem. . 372 . 2 . 198–203 . January 2008 . 17920029 . 10.1016/j.ab.2007.08.016 .
- Gary RK, Kindell SM . Quantitative assay of senescence-associated beta-galactosidase activity in mammalian cell extracts . Anal. Biochem. . 343 . 2 . 329–34 . August 2005 . 16004951 . 10.1016/j.ab.2005.06.003 .
- Book: Itahana K, Campisi J, Dimri GP . Methods to Detect Biomarkers of Cellular Senescence . Biological Aging . 371 . 21–31 . 2007 . 17634571 . 10.1007/978-1-59745-361-5_3 . https://archive.org/details/biologicalagingm00toll/page/21 . Methods in Molecular Biology . Humana Press . 978-1-58829-658-0 . registration .
- Lee BY, Han JA, Im JS, Morrone A, Johung K, Goodwin EC, Kleijer WJ, DiMaio D, Hwang ES . Senescence-associated beta-galactosidase is lysosomal beta-galactosidase . Aging Cell . 5 . 2 . 187–95 . April 2006 . 16626397 . 10.1111/j.1474-9726.2006.00199.x . 2158/216175 . 82432911 . free .
- Lee BY, Han JA, Im JS, Morrone A, Johung K, Goodwin EC, Kleijer WJ, DiMaio D, Hwang ES . Senescence-associated beta-galactosidase is lysosomal beta-galactosidase . Aging Cell . 5 . 2 . 187–95 . April 2006 . 16626397 . 10.1111/j.1474-9726.2006.00199.x . 2158/216175 . 82432911 . free .
