| Competition: | Peruvian Segunda División |
| Season: | 2001 |
| Dates: | 5 May – 8 December 2001 |
| Winners: | Alcides Vigo |
| Relegated: | Hijos de Yurimaguas Aurora Chancayllo |
| Prevseason: | 2000 |
| Nextseason: | 2002 |
The 2001 Peruvian Segunda División, the second division of Peruvian football (soccer), was played by 16 teams. The tournament winner, Alcides Vigo was promoted to the Playoff. The last places, Aurora Chancayllo and Hijos de Yurimaguas were relegated. The tournament was played on a home-and-away round-robin basis.
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