Ololygon luizotavioi is a species of frog in the family Hylidae. It is endemic to Brazil.
Its natural habitats are subtropical or tropical moist lowland forests, subtropical or tropical moist montane forests, moist savanna, subtropical or tropical moist shrubland, subtropical or tropical dry lowland grassland, subtropical or tropical high-altitude grassland, rivers, freshwater marshes, intermittent freshwater marshes, pastureland, rural gardens, ponds, and canals and ditches.It is threatened by habitat loss.