Rubroboletus satanas explained

Rubroboletus satanas, commonly known as Satan's bolete or the Devil's bolete, is a basidiomycete fungus of the bolete family (Boletaceae) and one of its most infamous members. It was known as Boletus satanas before its transfer to the new genus Rubroboletus in 2014, based on molecular phylogenetic data. Found in broad-leaved and mixed woodland in the warmer regions of Europe, it is classified as a poisonous mushroom, known to cause violent gastroenteritis. However, reports of poisoning are rare, due to the striking coloration and unpleasant odor of the fruiting bodies, which discourage experimentation.

These squat, brightly coloured fruiting bodies are often massive and imposing, with a beige-coloured velvet-textured cap up to 50cm (20inches) across, yellow to orange-red pores and a bulbous red stem. The flesh turns blue when cut or bruised, and fruit bodies often emit an unpleasant rotten odor. It is arguably the largest bolete found in Europe.

Taxonomy and phylogeny

Originally known as Boletus satanas, the Satan's bolete was described by German mycologist Harald Othmar Lenz in 1831. Lenz was aware of several reports of adverse reactions from people who had consumed this fungus and apparently felt himself ill from its "emanations" while describing it, hence giving it its sinister epithet.[1] The Greek word Greek, Ancient (to 1453);: σατανᾶς (satanas, meaning Satan), is derived from the Hebrew śāṭān (שטן). American mycologist Harry D. Thiers concluded that material from North America matches the species description,[2] however, genetic testing has since confirmed that western North American collections represent Rubroboletus eastwoodiae, a different species.

Genetic analysis published in 2013 revealed that B. satanas and several other red-pored boletes, are part of the "dupainii" clade (named after B. dupainii), and are distantly nested from the core group of Boletus (including B. edulis and relatives) within the Boletineae. This indicated that B. satanas and its relatives belonged to a distinct genus.[3] The species was hence transferred to the new genus Rubroboletus in 2014, along with several allied red-pored, blue-staining bolete species. Genetic testing on several species of the genus revealed that R. satanas is most closely related to R. pulchrotinctus, a morphologically similar but much rarer species occurring in the Mediterranean regions

Common names

Both Rubroboletus satanas and Suillellus luridus are known as ayimantari ('bear mushroom') in eastern Turkey.[4]

Description

The compact cap can reach an impressive 300NaN0, extraordinarily 400NaN0, very rarely 500NaN0 in diameter. At first it is hemispherical with an inrolled margin, but becomes convex at maturity as the fruit body expands, while in older specimens the margin might be slightly undulating. When young, the pileus is greyish white to silvery-white or buff, but older specimens tend to develop olivaceous, ochraceous or brownish tinges. The surface of the cap is finely tomentose, becoming smooth at maturity and is often slightly viscid in wet weather. The cuticle is tightly attached to the flesh and does not peel.

The free to slightly adnate tubes are up to 3frac=8NaNfrac=8 long, pale yellow or greenish yellow and bluing when cut. The pores (tube mouths) are rounded, yellow to orange at first, but soon turning red from the point of their attachment to the stem outwards, eventually becoming entirely purplish red or carmine-red at full maturity and instantly bluing when touched or bruised. The stipe is 5–, extraordinarily 20round=0.5NaNround=0.5, very rarely 25round=0.5NaNround=0.5 long, distinctly bulbous (4–, extraordinarily 16abbr=onNaNabbr=on, very rarely 20abbr=onNaNabbr=on), and often wider than its length, becoming more ventricose as the fungus expands but remaining bulbous at the base. Its colour is golden-yellow to orange at the apex, becoming increasingly pinkish-red to reddish-orange further down and deep carmine-red to purple-red towards the base. It is decorated in a fine, yellowish to reddish hexagonal net, sometimes confined to the upper half of the stipe. The flesh is thick, spongy and whitish, but may be yellow to straw-coloured in immature specimens and is sometimes reddish at the stem base. It slowly turns a faded blue colour when cut, bluing more intensely around the apex and above the tubes. The smell is weak and pleasantly musky in young fruit bodies, but becomes increasingly putrid in older specimens, reminiscent of carrion. Young specimens have a reportedly pleasant, nutty taste.[5] The spore print is olivaceous green.

The spores are fusiform (spindle-shaped) when viewed under a microscope and measure 10–16 × 4.5–7.5 μm. The cap cuticle is composed of interwoven septate hyphae, which are often finely incrusted.

Similar species

Satan's bolete can be confused with a number of other species:

Distribution and habitat

Rubroboletus satanas is widely distributed throughout the temperate zone, but is rare in most of its reported localities. In Europe, it mostly occurs in the southern regions and is rare or absent in northern countries. It fruits in the summer and early autumn in warm, broad-leaved and mixed forests, forming ectomycorrhizal associations with oak (Quercus) and sweet chestnut (Castanea), with a preference for calcareous (chalky) soils. Other frequently reported hosts are hornbeam (Carpinus), beech (Fagus) and lime and linden trees (Tilia).

In the United Kingdom, this striking bolete is found only in the south of England.[6] It is rare in Scandinavia, occurring primarily on a few islands in the Baltic Sea where conditions are favourable, with highly calcareous soil.[6] In the eastern Mediterranean region, it has been reported from the Bar'am Forest in the Upper Galilee region of northern Israel, as well as the island of Cyprus, where it is found in association with the narrow-endemic golden oak (Quercus alnifolia). It has further been documented in the Black Sea and eastern Anatolia regions of Turkey,[4] [7] as well as Crimea and Ukraine,[8] with its distribution possibly extending as far south as Iran.

In the past, R. satanas had been reported from the United States,[9] however, these sightings are instead of the closely related species Rubroboletus eastwoodiae.

Toxicity

This mushroom is moderately poisonous, especially if eaten raw. The symptoms, which are predominantly gastrointestinal in nature, include nausea, abdominal pain, and violent vomiting with bloody diarrhea that can last up to six hours.[10] [11] [12] [13] [14]

The toxic enzyme bolesatine has been isolated from fruiting bodies of R. satanas and is implicated in the poisonings.[15] Bolesatine is a protein synthesis inhibitor and, when given to mice, causes massive thrombosis.[16] At lower concentrations, bolesatine is a mitogen, inducing cell division in human T lymphocytes.[17] Muscarine has also been isolated from this fungus, but the quantities are believed to be far too small to cause toxic effects in humans. More recent studies have associated the poisoning caused by R. satanas with hyperprocalcitonemia,[18] and classified it as a distinct syndrome among fungal poisonings.[19]

Controversially, English mycologist John Ramsbottom reported in 1953 that R. satanas is consumed in certain parts of Italy and the former Czechoslovakia.[1] In those regions, the fungus is reportedly eaten following prolonged boiling that may neutralise the toxins, though this has never been proven scientifically. Similar reports exist from the San Francisco Bay Area of the United States,[2] but probably involve a different fungus misidentified as R. satanas. Ramsbottom speculated that there may be a regional variation in its toxicity, and conceded that the fungus may not be as poisonous as widely reported.[1] Nevertheless, R. satanas is rarely sampled casually, not least because of the foul smell, which in addition to their bright red colour and blue staining, make this fungus unappealing for human consumption.

Notes and References

  1. Book: Ramsbottom J . 1953 . Mushrooms & Toadstools . 53–54 . Collins . 1-870630-09-2.
  2. Book: California Mushrooms  - A Field Guide to the Boletes . Thiers HD . 1975 . Hafner Press . New York . 0-02-853410-7 . 58.
  3. Nuhn ME, Binder M, ((Taylor AFS)), Halling RE, Hibbett DS . . Fungal Biology . 2013 . 117 . 7–8 . 479–511 . 10.1016/j.funbio.2013.04.008. 23931115 .
  4. Demirel K, Uzun Y, Kaya A . 2004. Some Poisonous Fungi of East Anatolia . Turk J Bot . 28. 215–19 . 2008-02-08.
  5. Book: Zeitlmayr L. 1976. Wild Mushrooms:An Illustrated Handbook. Garden City Press, Hertfordshire. 0-584-10324-7. 102.
  6. Book: Nilson S, Persson O . 1977 . Fungi of Northern Europe 1: Larger Fungi (Excluding Gill-Fungi) . Penguin. 0-14-063005-8. 104.
  7. Sesli E . 2007. Preliminary checklist of macromycetes of the East and Middle Black Sea Regions of Turkey . Mycotaxon . 99. 71–74.
  8. Akulov . O.Yu . Pryidiuk . M.P. . https://www.researchgate.net/publication/323320519 The preliminary checklist of boletoid fungi of Ukraine ]. Pagine di Micologia . 2007 . 27 . 117–144 .
  9. Book: Poisonous Mushrooms of the Northern United States and Canada; . limited . Ammirati JA, Traquair JA, Horgen PA . 1985. University of Minnesota Press . Minneapolis . 0-8166-1407-5. 241–42.
  10. Book: North, Pamela . 1967 . Poisonous Plants and Fungi in colour . Blandford Press & Pharmacological Society of Great Britain .
  11. Brensinsky A, Besl H. (1990). A colour atlas of poisonous fungi. Wolfe Publishing Ltd, London. 295 p.
  12. Spoerke DG, Rumack BH (eds). (1994). Handbook of mushroom poisoning: diagnosis and treatment. CRC Press, Boca Raton.
  13. Berger KJ, Guss DA. (2005). Mycotoxins revisited: Part I. J Emerg Med 28:53.
  14. Schenk-Jaeger KM, Rauber-Lüthy C, Bodmer M, Kupferschmidt H, Kullak-Ublick GA, Ceschi A . 2012. Mushroom poisoning: a study on circumstances of exposure and patterns of toxicity. European Journal of Internal Medicine. 23. 4. e85–e91. 10.1016/j.ejim.2012.03.014. 22560399.
  15. Kretz O, Creppy EE, Dirheimer G . Characterization of bolesatine, a toxic protein from the mushroom Boletus satanas Lenz and its effects on kidney cells . Toxicology . 66 . 2 . 213–24 . 1991 . 1707561 . 10.1016/0300-483X(91)90220-U.
  16. Ennamany R, Bingen A, Creppy EE, Kretz O, Gut JP, Dubuisson L, Balabaud C, Sage PB, Kirn A . 1998 . Aspirin (R) and heparin prevent hepatic blood stasis and thrombosis induced by the toxic glycoprotein Bolesatine in mice . Human & Experimental Toxicology . 17 . 11 . 620 - 624 . 10.1191/096032798678908017 . 9865419 .
  17. Licastro F, Morini MC, Kretz O, Dirheimer G, Creppy EE; Stirpe F. (1993). "Mitogenic activity and immunological properties of bolesatine, a lectin isolated from the mushroom Boletus satanas Lenz". International Journal of Biochemistry. 25 (5): 789–792.
  18. Merlet A, Dauchy FA, Dupon M. (2012). Hyperprocalcitonemia due to mushroom poisoning. Clin Infect Dis. 54: 307–308.
  19. White J, Weinstein SA, De Haro L, Bédry R, Schaper A, Rumack BH, Zilker T. (2018). Mushroom poisoning: a proposed new clinical classification. Toxicon. doi: 10.1016/j.toxicon.2018.11.007