| Santiago Ayuquililla | |
| Settlement Type: | Municipality and town |
| Pushpin Map: | Mexico |
| Pushpin Label Position: | above |
| Pushpin Mapsize: | 300 |
| Pushpin Map Caption: | Location in Mexico |
| Subdivision Type: | Country |
| Subdivision Name: |  Mexico |
| Subdivision Type1: | State |
| Subdivision Name1: | Oaxaca |
| Population As Of: | 2005 |
| Timezone: | Central Standard Time |
| Utc Offset: | -6 |
| Timezone Dst: | Central Daylight Time |
| Utc Offset Dst: | -5 |
| Coordinates: | 17.95°N -155°W |
Santiago Ayuquililla is a town and municipality in Oaxaca in south-western Mexico. The municipality covers an area of 90.13 km2. It is part of the Huajuapan District in the north of the Mixteca Region.
As of 2010, the municipality had a total population of 2,748.[1]