| Santa Ana de Pusa | |
| Settlement Type: | Municipality |
| Pushpin Map: | Spain |
| Pushpin Map Caption: | Location in Spain |
| Coordinates: | 39.7636°N -4.7158°W |
| Subdivision Type: | Country |
| Subdivision Name: |  Spain |
| Subdivision Type1: | Community |
| Subdivision Type2: | Province |
| Subdivision Name2: | Toledo |
| Subdivision Type3: | Comarca |
| Subdivision Name3: | La Jara |
| Leader Title: | Mayor |
| Area Total Km2: | 19 |
| Elevation M: | 594 |
| Population Density Km2: | auto |
| Timezone1: | CET |
| Utc Offset1: | +1 |
| Timezone1 Dst: | CEST |
| Utc Offset1 Dst: | +2 |
| Postal Code Type: | Postal code |
Santa Ana de Pusa is a municipality located in the province of Toledo, Castile-La Mancha, Spain. According to the 2006 census (INE), the municipality has a population of 411 inhabitants.