| Honorific-Prefix: | The Hon |
| Samuel Brassington | |
| Office1: | Speaker of the Queensland Parliament |
| Term Start1: | 1 August 1944 |
| Term End1: | 4 October 1950 |
| Predecessor1: | Ted Hanson |
| Successor1: | Johnno Mann |
| Constituency1: | Fortitude Valley |
| Constituency Am2: | Balonne |
| Assembly2: | Queensland Legislative |
| Term Start2: | 6 August 1927 |
| Term End2: | 11 June 1932 |
| Predecessor2: | Edward Land |
| Successor2: | Seat abolished |
| Constituency Am3: | Fortitude Valley |
| Assembly3: | Queensland Legislative |
| Term Start3: | 15 July 1933 |
| Term End3: | 4 October 1950 |
| Predecessor3: | Thomas Wilson |
| Successor3: | Michael Brosnan |
| Birth Date: | 6 May 1901 |
| Birth Place: | Augathella, Queensland |
| Death Place: | Brisbane, Queensland |
| Restingplace: | Toowong Cemetery |
| Birthname: | Samuel John Brassington |
| Nationality: | Australian |
| Party: | Labor |
| Spouse: | Merle Eileen Booth |
| Occupation: | Union official |
Samuel John Brassington (6 May 1901 - 4 October 1950) was an Australian politician. He was a Labor member of the Legislative Assembly of Queensland, serving as the member for Balonne from 1927 to 1932 and for Fortitude Valley from 1933 to 1950. He served as Speaker of the Legislative Assembly of Queensland from 1944 to 1950.[1]
Upon his death in 1950, Brassington was accorded a State funeral[2] which was held at St Stephen's Cathedral and proceeded to Toowong Cemetery.[2] [3]