| Samsudin Osman | |
| Honorific-Prefix: | Yang Berbahagia Tan Sri Dato' Sri |
| Order: | 11th |
| Office: | Chief Secretary to the Government of Malaysia |
| Primeminister: | Mahathir Mohamad Abdullah Ahmad Badawi |
| Term Start: | 1 February 2001 |
| Term End: | 2 September 2006 |
| Predecessor: | Abdul Halim Ali |
| Successor: | Mohd Sidek Hassan |
| Office1: | 9th President of the International Islamic University Malaysia |
| 1Blankname1: | Chancellor |
| 1Namedata1: | Tunku Azizah Aminah Maimunah Iskandariah |
| Term Start1: | 1 July 2022 |
| Predecessor1: | Mohd Daud Bakar |
| Office2: | Chairman of the Employees Provident Fund (Malaysia) |
| 1Blankname2: | Chief Executive Officer |
| 1Namedata2: | Tunku Alizakri Raja Muhammad Alias |
| Term Start2: | 1 February 2007 |
| Deputy2: | Siti Zauyah Md Desa |
| Predecessor2: | Abdul Halim Ali |
| Successor2: | Ahmad Badri Mohd Zahir |
| Term End2: | 30 April 2020 |
| Birth Name: | Samsudin bin Osman |
| Birth Date: | 1946 3, df=yes |
| Birth Place: | Johor Bahru, Johor, Malayan Union (now Malaysia) |
| Education: | English College Johore Bahru Royal Military College |
Samsudin bin Osman (born 3 March 1946)[1] is a Malaysian former public servant who served the Malaysian Government from 1969 to 2006. His last office in the public service was Chief Secretary to the Government from 1 February 2001 to 2 September 2006. He is currently the President of the International Islamic University Malaysia, and Former Chairman of the Employees Provident Fund.
Prior to being Chief Secretary, Samsudin was the secretary-general for the Ministry of Domestic Trade and Consumer Affairs from 1994 and the Ministry of Home Affairs from 1996.
He obtained his Bachelor of Arts (Hons) from the University of Malaya and Master of Public Administration from Pennsylvania State University. He is also a former student of the renowned English College Johore Bahru and Royal Military College, Sungai Besi.