Sakaguchi test explained

The Sakaguchi test is a chemical test used to detect presence of arginine in proteins. It is named after the Japanese food scientist and organic chemist, Shoyo Sakaguchi (1900 - 1995) who described the test in 1925.[1] The Sakaguchi reagent used in the test consists of 1-Naphthol and a drop of sodium hypobromite. The guanidino (–C group in arginine reacts with the Sakaguchi reagent to form a red-coloured complex.[2] [3] [4] [5]

Notes and References

  1. Sakaguchi . Shoyo . April 1925 . Über eine neue Farbenreaktion von protein und arginin . About a new colorimetric reaction of protein and arginine . . de . Oxford University Press. 5 . 25–31 . 10.1093/oxfordjournals.jbchem.a128359.
  2. Book: Chatterjea. Textbook of Biochemistry for Dental/Nursing/Pharmacy Students. 1 January 2004. Jaypee Brothers Publishers. 978-81-8061-204-6. 51.
  3. Book: R.A. Joshi. Question Bank of Biochemistry. 2006. New Age International. 978-81-224-1736-4. 64.
  4. Book: Dandekar. Practicals And Viva In Medical Biochemistry. 1 January 2004. Elsevier India. 978-81-8147-025-6. 28.
  5. Book: Joshi . Rashmi A. . Guide to Bio-chemistry . Marks . Victor . 30 June 2004 . . 9788180560538 . 74 . en.

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