Sadashiva Karnik Explained

Sadashiva "Sadu" Karnik is an Indian-born American molecular biologist who is a Professor in the Molecular Medicine Department of Cleveland Clinic Lerner College of Medicine at Case Western Reserve University. He is the Principal Investigator of the Sadashiva Karnik Laboratory, at the Lerner Research Institute of Cleveland Clinic.[1]

Early life and education

Sadu Karnik earned a B.Sc. degree in botany, zoology and chemistry from Sri Poornaprajna College, Udupi in 1973 and a M.Sc. degree in Clinical Biochemistry by research from Kasturba Medical College, Manipal. He entered the Indian Institute of Science, Department of Microbiology and Cell Biology Bengaluru in 1976 for doctoral studies on Mycobacteriophage in the laboratory of Prof. K.P Gopinathan.[2] His post-doctoral studies were on Bacteriophage Qβ at the laboratory of molecular biologist Martin Billetter of the ETH Institute for Molecular Biology, University of Zurich, Switzerland.[3] He later joined the laboratory of Har Gobind Khorana at Massachusetts Institute of Technology to study disulfide-bonding in integral membrane proteins. He became a naturalized citizen of the United States in 1995.[4]

Career

Karnik started his independent laboratory at the Cleveland Clinic research foundation as assistant staff studying Angiotensin receptor molecular biology and was promoted to full-staff in 2002. In 2000 he received conjoint appointment as professor of chemistry and biology at Cleveland State University (CSU), Cleveland, OH, and in 2003, he became Professor (non-tenure track) of Molecular Medicine in the newly accredited Cleveland Clinic Lerner College of Medicine (CCLCM) at Case Western Reserve University, Cleveland, Ohio.[1] His research programs have been supported by National Institutes of Health funding continuously for the past 25 years.[5]

Research

The Karnik Laboratory is known for first reporting of constitutive activation, atypical G protein coupling,[6] biased ligand signaling,[7] X-ray structure of antihypertensive drug bound angiotensin[8] receptor,[9] and structure-based allosteric ligand of Angiotensin receptors.[10] These discoveries facilitated the development of novel transgenic models of cardiovascular diseases, β-arrestin biased agonists,[11] next generation antihypertensive drugs and novel structures of GPCRs.[9] His current research is aimed at developing novel allosteric ligand drugs for intervention in Preeclampsia due to autoimmunity and Hyperaldosteronism due to adrenal hyperplasia. In 1986, Karnik and colleagues at MIT reported that production of a functional, light sensing-state of rhodopsin depended on formation of a unique disulfide bond that is conserved in >90% GPCRs.[12] [13] The Karnik Laboratory at Cleveland Clinic extended this finding to other hormone and neurotransmitter GPCRs including β-adrenegic and angiotensin receptors.

Awards and honors

Karnik won several fellowships including the Junior Research Fellowship of the Indian Institute of Science, Department of Science Technology (India) Senior Research Fellowship and the Swiss National Science Foundation Fellowship.[14]

Selected bibliography

Notes and References

  1. Web site: Sadashiva Karnik Laboratory . 2024-04-02 . Cleveland Clinic, Lerner Research Institute.
  2. Karnik. S. S.. Gopinathan. K. P.. 1980-03-01. Possible involvement of a calcium-stimulated ATP-hydrolyzing activity associated with mycobacteriophage I3 in the DNA injection process.. Journal of Virology. en. 33. 3. 969–975. 10.1128/JVI.33.3.969-975.1980. 0022-538X. 6445012. 288630.
  3. Karnik. S.. Billeter. M.. The lysis function of RNA bacteriophage Qbeta is mediated by the maturation (A2) protein.. The EMBO Journal. 1983. en. 2. 9. 1521–1526. 10.1002/j.1460-2075.1983.tb01617.x. 11892805. 555316.
  4. Karnik. Sadashiva. Subramaniam. Sriram. 2012-12-01. There is no overkill in biochemistry. Resonance. en. 17. 12. 1157–1164. 10.1007/s12045-012-0132-6. 80667485. 0973-712X.
  5. Web site: NIH Awards by Location and Organization - NIH Research Portfolio Online Reporting Tools (RePORT). 2020-12-08. www.report.nih.gov.
  6. Tirupula. Kalyan C.. Desnoyer. Russell. Speth. Robert C.. Karnik. Sadashiva S.. 2014. Atypical signaling and functional desensitization response of MAS receptor to peptide ligands. PLOS ONE. 9. 7. e103520. 10.1371/journal.pone.0103520. 1932-6203. 4113456. 25068582. 2014PLoSO...9j3520T. free.
  7. Web site: Biased Ligands - an overview ScienceDirect Topics. 2020-12-08. www.sciencedirect.com.
  8. Noda. K.. Feng. Y. H.. Liu. X. P.. Saad. Y.. Husain. A.. Karnik. S. S.. 1996-12-24. The active state of the AT1 angiotensin receptor is generated by angiotensin II induction. Biochemistry. 35. 51. 16435–16442. 10.1021/bi961593m. 0006-2960. 8987975.
  9. Singh. Khuraijam Dhanachandra. Unal. Hamiyet. Desnoyer. Russell. Karnik. Sadashiva S.. Divergent Spatiotemporal Interaction of Angiotensin Receptor Blocking Drugs with Angiotensin Type 1 Receptor. Journal of Chemical Information and Modeling. 2018. 58. 1. 182–193. 10.1021/acs.jcim.7b00424. 1549-960X. 6058968. 29195045.
  10. Zhang. Haitao. Unal. Hamiyet. Gati. Cornelius. Han. Gye Won. Liu. Wei. Zatsepin. Nadia A.. James. Daniel. Wang. Dingjie. Nelson. Garrett. Weierstall. Uwe. Sawaya. Michael R.. Structure of the Angiotensin Receptor Revealed by Serial Femtosecond Crystallography. Cell. 2015. 161. 4. 833–844. 10.1016/j.cell.2015.04.011. 0092-8674. 4427029. 25913193.
  11. Zanaty. Mario. Seara. Fernando A. C.. Nakagawa. Pablo. Deng. Guorui. Mathieu. Natalia M.. Balapattabi. Kirthikaa. Karnik. Sadashiva S.. Grobe. Justin L.. Sigmund. Curt D.. 2020-11-30. β-Arrestin-Biased Agonist Targeting the Brain AT1R (Angiotensin II Type 1 Receptor) Increases Aversion to Saline and Lowers Blood Pressure in Deoxycorticosterone Acetate-Salt Hypertension. Hypertension. 77. 2. 420–431. 10.1161/HYPERTENSIONAHA.120.15793. 1524-4563. 33249862. 7855825.
  12. Unal. Hamiyet. Jagannathan. Rajaganapathi. Bhatnagar. Anushree. Tirupula. Kalyan. Desnoyer. Russell. Karnik. Sadashiva S.. 2013-01-04. Long Range Effect of Mutations on Specific Conformational Changes in the Extracellular Loop 2 of Angiotensin II Type 1 Receptor. Journal of Biological Chemistry. en. 288. 1. 540–551. 10.1074/jbc.M112.392514. 0021-9258. 3537051. 23139413. free.
  13. Karnik. S. Gogonea. C. Patil. S. Saad. Y. Takezako. T. November 2003. Activation of G-protein-coupled receptors: a common molecular mechanism. Trends in Endocrinology and Metabolism. en. 14. 9. 431–437. 10.1016/j.tem.2003.09.007. 14580763. 6956555.
  14. Web site: Speaker Biographies. 2020-12-08. Drug Discovery Chemistry. en.

This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Sadashiva Karnik".

Except where otherwise indicated, Everything.Explained.Today is © Copyright 2009-2024, A B Cryer, All Rights Reserved. Cookie policy.