SYTL2 explained

Synaptotagmin-like 2, also known as SYTL2, is a human gene.^[1]

Function

The protein encoded by this gene is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. The SLP homology domain (SHD) of this protein has been shown to specifically bind the GTP-bound form of Ras-related protein Rab-27A (RAB27A), which suggests a role in vesicle trafficking. Multiple alternatively spiced transcript variants encoding distinct isoforms have been observed.

Interactions

SYTL2 has been shown to interact with RAB27A.^{[2] [3] [4]}

Further reading

• Nalefski EA, Falke JJ . The C2 domain calcium-binding motif: structural and functional diversity . Protein Science . 5 . 12 . 2375–90 . December 1996 . 8976547 . 2143302 . 10.1002/pro.5560051201 .
• Duncan RR, Shipston MJ, Chow RH . Double C2 protein. A review . Biochimie . 82 . 5 . 421–6 . May 2000 . 10865129 . 10.1016/S0300-9084(00)00214-5 .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . January 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . October 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Dias Neto E, Correa RG, Verjovski-Almeida S, Briones MR, Nagai MA, da Silva W, Zago MA, Bordin S, Costa FF, Goldman GH, Carvalho AF, Matsukuma A, Baia GS, Simpson DH, Brunstein A, de Oliveira PS, Bucher P, Jongeneel CV, O'Hare MJ, Soares F, Brentani RR, Reis LF, de Souza SJ, Simpson AJ . Shotgun sequencing of the human transcriptome with ORF expressed sequence tags . Proceedings of the National Academy of Sciences of the United States of America . 97 . 7 . 3491–6 . March 2000 . 10737800 . 16267 . 10.1073/pnas.97.7.3491 . 2000PNAS...97.3491D . free .
• Nagase T, Kikuno R, Nakayama M, Hirosawa M, Ohara O . Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro . DNA Research . 7 . 4 . 273–81 . August 2000 . 10997877 . 10.1093/dnares/7.4.271 . free .
• Fukuda M, Mikoshiba K . Synaptotagmin-like protein 1-3: a novel family of C-terminal-type tandem C2 proteins . Biochemical and Biophysical Research Communications . 281 . 5 . 1226–33 . March 2001 . 11243866 . 10.1006/bbrc.2001.4512 .
• Fukuda M, Saegusa C, Mikoshiba K . Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: identification of the Slp homology domain . Biochemical and Biophysical Research Communications . 283 . 2 . 513–9 . May 2001 . 11327731 . 10.1006/bbrc.2001.4803 .
• Craxton M . Genomic analysis of synaptotagmin genes . Genomics . 77 . 1–2 . 43–9 . September 2001 . 11543631 . 10.1006/geno.2001.6619 .
• Kuroda TS, Fukuda M, Ariga H, Mikoshiba K . The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain . The Journal of Biological Chemistry . 277 . 11 . 9212–8 . March 2002 . 11773082 . 10.1074/jbc.M112414200 . free .
• Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proceedings of the National Academy of Sciences of the United States of America . 99 . 26 . 16899–903 . December 2002 . 12477932 . 139241 . 10.1073/pnas.242603899 . 2002PNAS...9916899M . free .
• Fukuda M . Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells . The Journal of Biological Chemistry . 278 . 17 . 15390–6 . April 2003 . 12590134 . 10.1074/jbc.M213090200 . free .
• Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM . Functional proteomics mapping of a human signaling pathway . Genome Research . 14 . 7 . 1324–32 . July 2004 . 15231748 . 442148 . 10.1101/gr.2334104 .
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . October 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
• Otsuki T, Ota T, Nishikawa T, Hayashi K, Suzuki Y, Yamamoto J, Wakamatsu A, Kimura K, Sakamoto K, Hatano N, Kawai Y, Ishii S, Saito K, Kojima S, Sugiyama T, Ono T, Okano K, Yoshikawa Y, Aotsuka S, Sasaki N, Hattori A, Okumura K, Nagai K, Sugano S, Isogai T . Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries . DNA Research . 12 . 2 . 117–26 . 2007 . 16303743 . 10.1093/dnares/12.2.117 . free .
• Yu M, Kasai K, Nagashima K, Torii S, Yokota-Hashimoto H, Okamoto K, Takeuchi T, Gomi H, Izumi T . Exophilin4/Slp2-a targets glucagon granules to the plasma membrane through unique Ca2+-inhibitory phospholipid-binding activity of the C2A domain . Molecular Biology of the Cell . 18 . 2 . 688–96 . February 2007 . 17182843 . 1783794 . 10.1091/mbc.E06-10-0914 .

Notes and References

1. Web site: Entrez Gene: SYTL2 synaptotagmin-like 2.
2. Fukuda M . Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells . The Journal of Biological Chemistry . 278 . 17 . 15390–6 . April 2003 . 12590134 . 10.1074/jbc.M213090200 . free .
3. Strom M, Hume AN, Tarafder AK, Barkagianni E, Seabra MC . A family of Rab27-binding proteins. Melanophilin links Rab27a and myosin Va function in melanosome transport . The Journal of Biological Chemistry . 277 . 28 . 25423–30 . July 2002 . 11980908 . 10.1074/jbc.M202574200 . free .
4. Kuroda TS, Fukuda M, Ariga H, Mikoshiba K . The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain . The Journal of Biological Chemistry . 277 . 11 . 9212–8 . March 2002 . 11773082 . 10.1074/jbc.M112414200 . free .