SSX2IP explained

Afadin- and alpha-actinin-binding protein is a protein that in humans is encoded by the SSX2IP gene.^[1] It has been shown that it functions together with WDR8 in centrosome maturation, ensuring proper spindle length and orientation.^{[2] [3] [4] [5] [6]} The SSX2IP-WDR8 complex additionally promotes ciliary vesicle docking during ciliogenesis.^{[7] [8]}

Interactions

SSX2IP has been initially described as a protein to interact with MLLT4^[9] and Actinin, alpha 1.^[9] In the context of centrosome maturation and ciliogenesis it has been shown to interact with WDR8.

Further reading

• Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O . Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro . DNA Research . 6 . 1 . 63–70 . February 1999 . 10231032 . 10.1093/dnares/6.1.63 . free .
• Hartley JL, Temple GF, Brasch MA . DNA cloning using in vitro site-specific recombination . Genome Research . 10 . 11 . 1788–95 . November 2000 . 11076863 . 310948 . 10.1101/gr.143000 .
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A . Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs . Genome Research . 11 . 3 . 422–35 . March 2001 . 11230166 . 311072 . 10.1101/gr.GR1547R .
• Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S . Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing . EMBO Reports . 1 . 3 . 287–92 . September 2000 . 11256614 . 1083732 . 10.1093/embo-reports/kvd058 .
• de Bruijn DR, dos Santos NR, Kater-Baats E, Thijssen J, van den Berk L, Stap J, Balemans M, Schepens M, Merkx G, van Kessel AG . The cancer-related protein SSX2 interacts with the human homologue of a Ras-like GTPase interactor, RAB3IP, and a novel nuclear protein, SSX2IP . Genes, Chromosomes & Cancer . 34 . 3 . 285–98 . July 2002 . 12007189 . 10.1002/gcc.10073 . 11734893 .
• Asada M, Irie K, Morimoto K, Yamada A, Ikeda W, Takeuchi M, Takai Y . ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions . The Journal of Biological Chemistry . 278 . 6 . 4103–11 . February 2003 . 12446711 . 10.1074/jbc.M209832200 . free .
• Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A . From ORFeome to biology: a functional genomics pipeline . Genome Research . 14 . 10B . 2136–44 . October 2004 . 15489336 . 528930 . 10.1101/gr.2576704 .
• Guinn BA, Bland EA, Lodi U, Liggins AP, Tobal K, Petters S, Wells JW, Banham AH, Mufti GJ . Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia . Biochemical and Biophysical Research Communications . 335 . 4 . 1293–304 . October 2005 . 16112646 . 10.1016/j.bbrc.2005.08.024 .
• Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S . The LIFEdb database in 2006 . Nucleic Acids Research . 34 . Database issue . D415-8 . January 2006 . 16381901 . 1347501 . 10.1093/nar/gkj139 .
• Nousiainen M, Silljé HH, Sauer G, Nigg EA, Körner R . Phosphoproteome analysis of the human mitotic spindle . Proceedings of the National Academy of Sciences of the United States of America . 103 . 14 . 5391–6 . April 2006 . 16565220 . 1459365 . 10.1073/pnas.0507066103 . 2006PNAS..103.5391N . free .

Notes and References

1. Web site: Entrez Gene: SSX2IP synovial sarcoma, X breakpoint 2 interacting protein.
2. Bärenz F, Inoue D, Yokoyama H, Tegha-Dunghu J, Freiss S, Draeger S, Mayilo D, Cado I, Merker S, Klinger M, Hoeckendorf B, Pilz S, Hupfeld K, Steinbeisser H, Lorenz H, Ruppert T, Wittbrodt J, Gruss OJ . 6 . The centriolar satellite protein SSX2IP promotes centrosome maturation . The Journal of Cell Biology . 202 . 1 . 81–95 . July 2013 . 23816619 . 10.1083/jcb.201302122 . 3704989 .
3. Yukawa M, Ikebe C, Toda T . The Msd1-Wdr8-Pkl1 complex anchors microtubule minus ends to fission yeast spindle pole bodies . The Journal of Cell Biology . 209 . 4 . 549–62 . May 2015 . 25987607 . 4442821 . 10.1083/jcb.201412111 .
4. Inoue D, Stemmer M, Thumberger T, Ruppert T, Bärenz F, Wittbrodt J, Gruss OJ . Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses . En . Nature Communications . 8 . 14090 . January 2017 . 28098238 . 10.1038/ncomms14090 . 5253655 . 2017NatCo...814090I .
5. Hori A, Morand A, Ikebe C, Frith D, Snijders AP, Toda T . The conserved Wdr8-hMsd1/SSX2IP complex localises to the centrosome and ensures proper spindle length and orientation . Biochemical and Biophysical Research Communications . 468 . 1–2 . 39–45 . 2015-12-04 . 26545777 . 10.1016/j.bbrc.2015.10.169 . 4669408 .
6. Hori A, Peddie CJ, Collinson LM, Toda T . Centriolar satellite- and hMsd1/SSX2IP-dependent microtubule anchoring is critical for centriole assembly . Molecular Biology of the Cell . 26 . 11 . 2005–19 . June 2015 . 25833712 . 4472012 . 10.1091/mbc.E14-11-1561 .
7. Kurtulmus B, Wang W, Ruppert T, Neuner A, Cerikan B, Viol L, Dueñas-Sánchez R, Gruss OJ, Pereira G . WDR8 is a centriolar satellite and centriole-associated protein that promotes ciliary vesicle docking during ciliogenesis . Journal of Cell Science . 129 . 3 . 621–36 . February 2016 . 26675238 . 10.1242/jcs.179713 . free .
8. Klinger M, Wang W, Kuhns S, Bärenz F, Dräger-Meurer S, Pereira G, Gruss OJ . The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone . Molecular Biology of the Cell . 25 . 4 . 495–507 . February 2014 . 24356449 . 10.1091/mbc.E13-09-0526 . 3923641 .
9. Asada M, Irie K, Morimoto K, Yamada A, Ikeda W, Takeuchi M, Takai Y . ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions . The Journal of Biological Chemistry . 278 . 6 . 4103–11 . February 2003 . 12446711 . 10.1074/jbc.M209832200 . free .