Small nuclear ribonucleoprotein D2 explained

Small nuclear ribonucleoprotein Sm D2 is a protein that in humans is encoded by the SNRPD2 gene.^{[1] [2] [3]} It belongs to the small nuclear ribonucleoprotein core protein family, and is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified.

Interactions

Small nuclear ribonucleoprotein D2 has been shown to interact with DDX20,^[4] Small nuclear ribonucleoprotein D1,^{[5] [6]} Small nuclear ribonucleoprotein polypeptide F,^[5] CDC5L^[7] and SMN1.^[8]

Further reading

• Rokeach LA, Haselby JA, Hoch SO . Molecular cloning of a cDNA encoding the human Sm-D autoantigen. . Proc. Natl. Acad. Sci. U.S.A. . 85 . 13 . 4832–6 . 1988 . 3260384 . 10.1073/pnas.85.13.4832 . 280530 . 1988PNAS...85.4832R . free .
• Liu Q, Fischer U, Wang F, Dreyfuss G . The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins. . Cell . 90 . 6 . 1013–21 . 1997 . 9323129 . 10.1016/S0092-8674(00)80367-0 . 10855404 . free .
• Fury MG, Zhang W, Christodoulopoulos I, Zieve GW . Multiple protein: protein interactions between the snRNP common core proteins. . Exp. Cell Res. . 237 . 1 . 63–9 . 1998 . 9417867 . 10.1006/excr.1997.3750 .
• Kambach C, Walke S, Young R, etal . Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs. . Cell . 96 . 3 . 375–87 . 1999 . 10025403 . 10.1016/S0092-8674(00)80550-4 . 17379935 . free .
• Charroux B, Pellizzoni L, Perkinson RA, etal . Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems. . J. Cell Biol. . 147 . 6 . 1181–94 . 2000 . 10601333 . 10.1083/jcb.147.6.1181 . 2168095 .
• Charroux B, Pellizzoni L, Perkinson RA, etal . Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli. . J. Cell Biol. . 148 . 6 . 1177–86 . 2000 . 10725331 . 10.1083/jcb.148.6.1177 . 2174312 .
• Meister G, Bühler D, Laggerbauer B, etal . Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins. . Hum. Mol. Genet. . 9 . 13 . 1977–86 . 2000 . 10942426 . 10.1093/hmg/9.13.1977 . free .
• Ajuh P, Kuster B, Panov K, etal . Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry. . EMBO J. . 19 . 23 . 6569–81 . 2001 . 11101529 . 10.1093/emboj/19.23.6569 . 305846 .
• Selenko P, Sprangers R, Stier G, etal . SMN tudor domain structure and its interaction with the Sm proteins. . Nat. Struct. Biol. . 8 . 1 . 27–31 . 2001 . 11135666 . 10.1038/83014 . 27071310 .
• Urlaub H, Raker VA, Kostka S, Lührmann R . Sm protein-Sm site RNA interactions within the inner ring of the spliceosomal snRNP core structure. . EMBO J. . 20 . 1–2 . 187–96 . 2001 . 11226169 . 10.1093/emboj/20.1.187 . 140196 .
• Gubitz AK, Mourelatos Z, Abel L, etal . Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins. . J. Biol. Chem. . 277 . 7 . 5631–6 . 2002 . 11714716 . 10.1074/jbc.M109448200 . free .
• Pellizzoni L, Baccon J, Rappsilber J, etal . Purification of native survival of motor neurons complexes and identification of Gemin6 as a novel component. . J. Biol. Chem. . 277 . 9 . 7540–5 . 2002 . 11748230 . 10.1074/jbc.M110141200 . free .
• Friesen WJ, Wyce A, Paushkin S, etal . A novel WD repeat protein component of the methylosome binds Sm proteins. . J. Biol. Chem. . 277 . 10 . 8243–7 . 2002 . 11756452 . 10.1074/jbc.M109984200 . free .
• Fong YW, Zhou Q . Stimulatory effect of splicing factors on transcriptional elongation. . Nature . 414 . 6866 . 929–33 . 2002 . 11780068 . 10.1038/414929a . 1518837 .
• Jurica MS, Licklider LJ, Gygi SR, etal . Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. . RNA . 8 . 4 . 426–39 . 2002 . 11991638 . 10.1017/S1355838202021088 . 1370266 .
• Baccon J, Pellizzoni L, Rappsilber J, etal . Identification and characterization of Gemin7, a novel component of the survival of motor neuron complex. . J. Biol. Chem. . 277 . 35 . 31957–62 . 2002 . 12065586 . 10.1074/jbc.M203478200 . free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Blagoev B, Kratchmarova I, Ong SE, etal . A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling. . Nat. Biotechnol. . 21 . 3 . 315–8 . 2003 . 12577067 . 10.1038/nbt790 . 26838266 .

Notes and References

1. Lehmeier T, Raker V, Hermann H, Luhrmann R . cDNA cloning of the Sm proteins D2 and D3 from human small nuclear ribonucleoproteins: evidence for a direct D1-D2 interaction . Proc Natl Acad Sci U S A . 91 . 25 . 12317–21 . January 1995 . 7527560 . 45428 . 10.1073/pnas.91.25.12317 . 1994PNAS...9112317L . free .
2. Lehmeier T, Foulaki K, Luhrmann R . Evidence for three distinct D proteins, which react differentially with anti-Sm autoantibodies, in the cores of the major snRNPs U1, U2, U4/U6 and U5 . Nucleic Acids Res . 18 . 22 . 6475–84 . January 1991 . 1701240 . 332598 . 10.1093/nar/18.22.6475 .
3. Web site: Entrez Gene: SNRPD2 small nuclear ribonucleoprotein D2 polypeptide 16.5kDa.
4. 10.1083/jcb.147.6.1181 . Charroux . B . Pellizzoni L . Perkinson R A . Shevchenko A . Mann M . Dreyfuss G . December 1999 . Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems . J. Cell Biol. . 147 . 6 . 1181–94 . UNITED STATES. 0021-9525. 10601333 . 2168095 .
5. Fury . M G . Zhang W . Christodoulopoulos I . Zieve G W . November 1997 . Multiple protein: protein interactions between the snRNP common core proteins . Exp. Cell Res. . 237 . 1 . 63–9 . UNITED STATES. 0014-4827. 9417867 . 10.1006/excr.1997.3750 .
6. 10.1016/S0092-8674(00)80550-4 . Kambach . C . Walke S . Young R . Avis J M . de la Fortelle E . Raker V A . Lührmann R . Li J . Nagai K . February 1999 . Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs . Cell . 96 . 3 . 375–87 . UNITED STATES. 0092-8674. 10025403 . 17379935 . free .
7. Ajuh . P . Kuster B . Panov K . Zomerdijk J C . Mann M . Lamond A I . December 2000 . Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry . EMBO J. . 19 . 23 . 6569–81 . ENGLAND. 0261-4189. 11101529 . 10.1093/emboj/19.23.6569 . 305846 .
8. 10.1093/hmg/9.13.1977 . Meister . G . Bühler D . Laggerbauer B . Zobawa M . Lottspeich F . Fischer U . August 2000 . Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins . Hum. Mol. Genet. . 9 . 13 . 1977–86 . ENGLAND. 0964-6906. 10942426 . free .