SMARCA5 explained

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 is a protein that in humans is encoded by the SMARCA5 gene.^{[1] [2]}

Function

The protein encoded by this gene is a member of the ISWI family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.

Interactions

SMARCA5 has been shown to interact with RAD21,^[3] Histone deacetylase 2,^[3] POLE3,^[4] SATB1^[5] and BAZ1A.^{[3] [4] [5] [6] [7]}

Further reading

• Dirscherl SS, Krebs JE . Functional diversity of ISWI complexes . Biochemistry and Cell Biology . 82 . 4 . 482–9 . Aug 2004 . 15284901 . 10.1139/o04-044 .
• LeRoy G, Orphanides G, Lane WS, Reinberg D . Requirement of RSF and FACT for transcription of chromatin templates in vitro . Science . 282 . 5395 . 1900–4 . Dec 1998 . 9836642 . 10.1126/science.282.5395.1900 .
• Bochar DA, Savard J, Wang W, Lafleur DW, Moore P, Côté J, Shiekhattar R . A family of chromatin remodeling factors related to Williams syndrome transcription factor . Proceedings of the National Academy of Sciences of the United States of America . 97 . 3 . 1038–43 . Feb 2000 . 10655480 . 15513 . 10.1073/pnas.97.3.1038 . 2000PNAS...97.1038B . free .
• Poot RA, Dellaire G, Hülsmann BB, Grimaldi MA, Corona DF, Becker PB, Bickmore WA, Varga-Weisz PD . HuCHRAC, a human ISWI chromatin remodelling complex contains hACF1 and two novel histone-fold proteins . The EMBO Journal . 19 . 13 . 3377–87 . Jul 2000 . 10880450 . 313940 . 10.1093/emboj/19.13.3377 .
• Stopka T, Zakova D, Fuchs O, Kubrova O, Blafkova J, Jelinek J, Necas E, Zivny J . Chromatin remodeling gene SMARCA5 is dysregulated in primitive hematopoietic cells of acute leukemia . Leukemia . 14 . 7 . 1247–52 . Jul 2000 . 10914549 . 10.1038/sj.leu.2401807 . 7991196 .
• Aalfs JD, Narlikar GJ, Kingston RE . Functional differences between the human ATP-dependent nucleosome remodeling proteins BRG1 and SNF2H . The Journal of Biological Chemistry . 276 . 36 . 34270–8 . Sep 2001 . 11435432 . 10.1074/jbc.M104163200 . free .
• Strohner R, Nemeth A, Jansa P, Hofmann-Rohrer U, Santoro R, Längst G, Grummt I . NoRC--a novel member of mammalian ISWI-containing chromatin remodeling machines . The EMBO Journal . 20 . 17 . 4892–900 . Sep 2001 . 11532953 . 125270 . 10.1093/emboj/20.17.4892 .
• Bozhenok L, Wade PA, Varga-Weisz P . WSTF-ISWI chromatin remodeling complex targets heterochromatic replication foci . The EMBO Journal . 21 . 9 . 2231–41 . May 2002 . 11980720 . 125993 . 10.1093/emboj/21.9.2231 .
• Levine SS, Weiss A, Erdjument-Bromage H, Shao Z, Tempst P, Kingston RE . The core of the polycomb repressive complex is compositionally and functionally conserved in flies and humans . Molecular and Cellular Biology . 22 . 17 . 6070–8 . Sep 2002 . 12167701 . 134016 . 10.1128/MCB.22.17.6070-6078.2002 .
• Hakimi MA, Bochar DA, Schmiesing JA, Dong Y, Barak OG, Speicher DW, Yokomori K, Shiekhattar R . A chromatin remodelling complex that loads cohesin onto human chromosomes . Nature . 418 . 6901 . 994–8 . Aug 2002 . 12198550 . 10.1038/nature01024 . 2002Natur.418..994H . 4344470 .
• Yasui D, Miyano M, Cai S, Varga-Weisz P, Kohwi-Shigematsu T . SATB1 targets chromatin remodelling to regulate genes over long distances . Nature . 419 . 6907 . 641–5 . Oct 2002 . 12374985 . 10.1038/nature01084 . 2002Natur.419..641Y . 25822700 .
• Collins N, Poot RA, Kukimoto I, García-Jiménez C, Dellaire G, Varga-Weisz PD . An ACF1-ISWI chromatin-remodeling complex is required for DNA replication through heterochromatin . Nature Genetics . 32 . 4 . 627–32 . Dec 2002 . 12434153 . 10.1038/ng1046 . 22545162 .
• Loyola A, Huang JY, LeRoy G, Hu S, Wang YH, Donnelly RJ, Lane WS, Lee SC, Reinberg D . Functional analysis of the subunits of the chromatin assembly factor RSF . Molecular and Cellular Biology . 23 . 19 . 6759–68 . Oct 2003 . 12972596 . 193931 . 10.1128/MCB.23.19.6759-6768.2003 .
• Shu H, Chen S, Bi Q, Mumby M, Brekken DL . Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line . Molecular & Cellular Proteomics . 3 . 3 . 279–86 . Mar 2004 . 14729942 . 10.1074/mcp.D300003-MCP200 . free .
• Obuse C, Yang H, Nozaki N, Goto S, Okazaki T, Yoda K . Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase . Genes to Cells . 9 . 2 . 105–20 . Feb 2004 . 15009096 . 10.1111/j.1365-2443.2004.00705.x . 21813024 . free .
• Geiman TM, Sankpal UT, Robertson AK, Zhao Y, Zhao Y, Robertson KD . DNMT3B interacts with hSNF2H chromatin remodeling enzyme, HDACs 1 and 2, and components of the histone methylation system . Biochemical and Biophysical Research Communications . 318 . 2 . 544–55 . May 2004 . 15120635 . 10.1016/j.bbrc.2004.04.058 .
• Geiman TM, Sankpal UT, Robertson AK, Chen Y, Mazumdar M, Heale JT, Schmiesing JA, Kim W, Yokomori K, Zhao Y, Robertson KD . Isolation and characterization of a novel DNA methyltransferase complex linking DNMT3B with components of the mitotic chromosome condensation machinery . Nucleic Acids Research . 32 . 9 . 2716–29 . 2004 . 15148359 . 419596 . 10.1093/nar/gkh589 .

Notes and References

1. Aihara T, Miyoshi Y, Koyama K, Suzuki M, Takahashi E, Monden M, Nakamura Y . Cloning and mapping of SMARCA5 encoding hSNF2H, a novel human homologue of Drosophila ISWI . Cytogenetics and Cell Genetics . 81 . 3–4 . 191–3 . October 1998 . 9730600 . 10.1159/000015027 . 46768416 .
2. Web site: Entrez Gene: SMARCA5 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5.
3. Hakimi MA, Bochar DA, Schmiesing JA, Dong Y, Barak OG, Speicher DW, Yokomori K, Shiekhattar R . A chromatin remodelling complex that loads cohesin onto human chromosomes . Nature . 418 . 6901 . 994–8 . Aug 2002 . 12198550 . 10.1038/nature01024 . 2002Natur.418..994H . 4344470 .
4. Poot RA, Dellaire G, Hülsmann BB, Grimaldi MA, Corona DF, Becker PB, Bickmore WA, Varga-Weisz PD . HuCHRAC, a human ISWI chromatin remodelling complex contains hACF1 and two novel histone-fold proteins . The EMBO Journal . 19 . 13 . 3377–87 . Jul 2000 . 10880450 . 313940 . 10.1093/emboj/19.13.3377 .
5. Yasui D, Miyano M, Cai S, Varga-Weisz P, Kohwi-Shigematsu T . SATB1 targets chromatin remodelling to regulate genes over long distances . Nature . 419 . 6907 . 641–5 . Oct 2002 . 12374985 . 10.1038/nature01084 . 2002Natur.419..641Y . 25822700 .
6. Bochar DA, Savard J, Wang W, Lafleur DW, Moore P, Côté J, Shiekhattar R . A family of chromatin remodeling factors related to Williams syndrome transcription factor . Proceedings of the National Academy of Sciences of the United States of America . 97 . 3 . 1038–43 . Feb 2000 . 10655480 . 15513 . 10.1073/pnas.97.3.1038 . 2000PNAS...97.1038B . free .
7. Bozhenok L, Wade PA, Varga-Weisz P . WSTF-ISWI chromatin remodeling complex targets heterochromatic replication foci . The EMBO Journal . 21 . 9 . 2231–41 . May 2002 . 11980720 . 125993 . 10.1093/emboj/21.9.2231 .