S-phase kinase-associated protein 1 explained

S-phase kinase-associated protein 1 is an enzyme that in humans is encoded by the SKP1 gene.[1] [2] [3]

This gene encodes a protein that is a member of the SCF ubiquitin ligase protein complex. It binds to F-box proteins (proteins containing an F-box motif), such as cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin dependent proteolysis.[4] The encoded protein also collaborates with a network of proteins to control beta-catenin levels and affects the activity level of beta-catenin dependent TCF transcription factors. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

Interactions

SKP1A has been shown to interact with:

Further reading

Notes and References

  1. Zhang H, Kobayashi R, Galaktionov K, Beach D . p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase . Cell . 82 . 6 . 915–25 . October 1995 . 7553852 . 10.1016/0092-8674(95)90271-6 . 16325791 . free .
  2. Demetrick DJ, Zhang H, Beach DH . Chromosomal mapping of the genes for the human CDK2/cyclin A-associated proteins p19 (SKP1A and SKP1B) and p45 (SKP2) . Cytogenet Cell Genet . 73 . 1–2 . 104–7 . July 1996 . 8646875 . 10.1159/000134318 .
  3. Web site: Entrez Gene: SKP1A S-phase kinase-associated protein 1A (p19A).
  4. Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SJ . SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. . Cell . 86 . 2 . 263–74 . 1996 . 8706131 . 10.1016/S0092-8674(00)80098-7 . 18387009 . free .
  5. Suzuki H, Chiba T, Suzuki T, Fujita T, Ikenoue T, Omata M, Furuichi K, Shikama H, Tanaka K . Homodimer of two F-box proteins betaTrCP1 or betaTrCP2 binds to IkappaBalpha for signal-dependent ubiquitination . J. Biol. Chem. . 275 . 4 . 2877–84 . January 2000 . 10644755 . 10.1074/jbc.275.4.2877. free .
  6. Margottin F, Bour SP, Durand H, Selig L, Benichou S, Richard V, Thomas D, Strebel K, Benarous R . A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif . Mol. Cell . 1 . 4 . 565–74 . March 1998 . 9660940 . 10.1016/s1097-2765(00)80056-8. free .
  7. Matsuzawa SI, Reed JC . Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses . Mol. Cell . 7 . 5 . 915–26 . May 2001 . 11389839 . 10.1016/s1097-2765(01)00242-8. free .
  8. Ayad NG, Rankin S, Murakami M, Jebanathirajah J, Gygi S, Kirschner MW . Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC . Cell . 113 . 1 . 101–13 . April 2003 . 12679038 . 10.1016/s0092-8674(03)00232-0. 14962502 . free . 10.1.1.522.1877 .
  9. Kiernan RE, Emiliani S, Nakayama K, Castro A, Labbé JC, Lorca T, Nakayama Ki K, Benkirane M . Interaction between cyclin T1 and SCF(SKP2) targets CDK9 for ubiquitination and degradation by the proteasome . Mol. Cell. Biol. . 21 . 23 . 7956–70 . Dec 2001 . 11689688 . 99964 . 10.1128/MCB.21.23.7956-7970.2001 .
  10. Staropoli JF, McDermott C, Martinat C, Schulman B, Demireva E, Abeliovich A . Parkin is a component of an SCF-like ubiquitin ligase complex and protects postmitotic neurons from kainate excitotoxicity . Neuron . 37 . 5 . 735–49 . March 2003 . 12628165 . 10.1016/s0896-6273(03)00084-9. 17024826 . free .
  11. Lisztwan J, Marti A, Sutterlüty H, Gstaiger M, Wirbelauer C, Krek W . Association of human CUL-1 and ubiquitin-conjugating enzyme CDC34 with the F-box protein p45(SKP2): evidence for evolutionary conservation in the subunit composition of the CDC34-SCF pathway . EMBO J. . 17 . 2 . 368–83 . January 1998 . 9430629 . 1170388 . 10.1093/emboj/17.2.368 .
  12. Zheng N, Schulman BA, Song L, Miller JJ, Jeffrey PD, Wang P, Chu C, Koepp DM, Elledge SJ, Pagano M, Conaway RC, Conaway JW, Harper JW, Pavletich NP . Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex . Nature . 416 . 6882 . 703–9 . April 2002 . 11961546 . 10.1038/416703a . 2002Natur.416..703Z . 4423882 .
  13. Ohta T, Michel JJ, Schottelius AJ, Xiong Y . ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity . Mol. Cell . 3 . 4 . 535–41 . April 1999 . 10230407 . 10.1016/s1097-2765(00)80482-7. 19371828 . free .
  14. Cenciarelli C, Chiaur DS, Guardavaccaro D, Parks W, Vidal M, Pagano M . Identification of a family of human F-box proteins . Curr. Biol. . 9 . 20 . 1177–9 . October 1999 . 10531035 . 10.1016/S0960-9822(00)80020-2 . 7467493 . free . 1999CBio....9.1177C .
  15. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D . Large-scale mapping of human protein-protein interactions by mass spectrometry . Mol. Syst. Biol. . 3 . 89 . 17353931 . 1847948 . 10.1038/msb4100134 . 2007.
  16. Wu G, Lyapina S, Das I, Li J, Gurney M, Pauley A, Chui I, Deshaies RJ, Kitajewski J . SEL-10 is an inhibitor of notch signaling that targets notch for ubiquitin-mediated protein degradation . Mol. Cell. Biol. . 21 . 21 . 7403–15 . November 2001 . 11585921 . 99913 . 10.1128/MCB.21.21.7403-7415.2001 .
  17. Min KW, Hwang JW, Lee JS, Park Y, Tamura TA, Yoon JB . TIP120A associates with cullins and modulates ubiquitin ligase activity . J. Biol. Chem. . 278 . 18 . 15905–10 . May 2003 . 12609982 . 10.1074/jbc.M213070200 . free .
  18. Strack P, Caligiuri M, Pelletier M, Boisclair M, Theodoras A, Beer-Romero P, Glass S, Parsons T, Copeland RA, Auger KR, Benfield P, Brizuela L, Rolfe M . SCF(beta-TRCP) and phosphorylation dependent ubiquitinationof I kappa B alpha catalyzed by Ubc3 and Ubc4 . Oncogene . 19 . 31 . 3529–36 . July 2000 . 10918611 . 10.1038/sj.onc.1203647 . 24267499 .
  19. Ng RW, Arooz T, Yam CH, Chan IW, Lau AW, Poon RY . Characterization of the cullin and F-box protein partner Skp1 . FEBS Lett. . 438 . 3 . 183–9 . November 1998 . 9827542 . 10.1016/s0014-5793(98)01299-x. 40950881 . free .
  20. Schulman BA, Carrano AC, Jeffrey PD, Bowen Z, Kinnucan ER, Finnin MS, Elledge SJ, Harper JW, Pagano M, Pavletich NP . Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex . Nature . 408 . 6810 . 381–6 . November 2000 . 11099048 . 10.1038/35042620 . 2000Natur.408..381S . 4300503 .
  21. Marti A, Wirbelauer C, Scheffner M, Krek W . Interaction between ubiquitin-protein ligase SCFSKP2 and E2F-1 underlies the regulation of E2F-1 degradation . Nat. Cell Biol. . 1 . 1 . 14–9 . May 1999 . 10559858 . 10.1038/8984 . 8884226 .