SF3B3 explained

Splicing factor 3B subunit 3 is a protein that in humans is encoded by the SF3B3 gene.^{[1] [2]}

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

Interactions

SF3B3 has been shown to interact with SF3B1,^[3] Transcription initiation protein SPT3 homolog^[4] and TAF9.^[4]

Further reading

• Nomura N, Miyajima N, Sazuka T, etal . Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 . DNA Res. . 1 . 1 . 27–35 . 1995 . 7584026 . 10.1093/dnares/1.1.27 . free .
• Nomura N, Miyajima N, Sazuka T, etal . Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 (supplement) . DNA Res. . 1 . 1 . 47–56 . 1995 . 7584028 . 10.1093/dnares/1.1.47 . free .
• Bonaldo MF . Lennon G . Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
• Mintz PJ, Patterson SD, Neuwald AF, etal . Purification and biochemical characterization of interchromatin granule clusters . EMBO J. . 18 . 15 . 4308–20 . 1999 . 10428969 . 10.1093/emboj/18.15.4308 . 1171507 .
• Brand M, Moggs JG, Oulad-Abdelghani M, etal . UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation . EMBO J. . 20 . 12 . 3187–96 . 2001 . 11406595 . 10.1093/emboj/20.12.3187 . 150203 .
• Gangloff YG, Pointud JC, Thuault S, etal . The TFIID Components Human TAFII140 and Drosophila BIP2 (TAFII155) Are Novel Metazoan Homologues of Yeast TAFII47 Containing a Histone Fold and a PHD Finger . Mol. Cell. Biol. . 21 . 15 . 5109–21 . 2001 . 11438666 . 10.1128/MCB.21.15.5109-5121.2001 . 87236 .
• Will CL, Schneider C, MacMillan AM, etal . A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site . EMBO J. . 20 . 16 . 4536–46 . 2001 . 11500380 . 10.1093/emboj/20.16.4536 . 125580 .
• Martinez E, Palhan VB, Tjernberg A, etal . Human STAGA Complex Is a Chromatin-Acetylating Transcription Coactivator That Interacts with Pre-mRNA Splicing and DNA Damage-Binding Factors In Vivo . Mol. Cell. Biol. . 21 . 20 . 6782–95 . 2001 . 11564863 . 10.1128/MCB.21.20.6782-6795.2001 . 99856 .
• Jurica MS, Licklider LJ, Gygi SR, etal . Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis . RNA . 8 . 4 . 426–39 . 2002 . 11991638 . 10.1017/S1355838202021088 . 1370266 .
• Scott MP, Zappacosta F, Kim EY, etal . Identification of novel SH3 domain ligands for the Src family kinase Hck. Wiskott-Aldrich syndrome protein (WASP), WASP-interacting protein (WIP), and ELMO1 . J. Biol. Chem. . 277 . 31 . 28238–46 . 2002 . 12029088 . 10.1074/jbc.M202783200 . free .
• Will CL, Urlaub H, Achsel T, etal . Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein . EMBO J. . 21 . 18 . 4978–88 . 2002 . 12234937 . 10.1093/emboj/cdf480 . 126279 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Cavusoglu N . Brand M . Tora L . Van Dorsselaer A . Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis . Proteomics . 3 . 2 . 217–23 . 2003 . 12601814 . 10.1002/pmic.200390030 . 6035986 .
• Li J, Hawkins IC, Harvey CD, etal . Regulation of Alternative Splicing by SRrp86 and Its Interacting Proteins . Mol. Cell. Biol. . 23 . 21 . 7437–47 . 2003 . 14559993 . 10.1128/MCB.23.21.7437-7447.2003 . 207616 .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Will CL, Schneider C, Hossbach M, etal . The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome . RNA . 10 . 6 . 929–41 . 2004 . 15146077 . 10.1261/rna.7320604 . 1370585 .
• Jin J, Smith FD, Stark C, etal . Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization . Curr. Biol. . 14 . 16 . 1436–50 . 2004 . 15324660 . 10.1016/j.cub.2004.07.051 . 2371325 . free .
• Gerhard DS, Wagner L, Feingold EA, etal . The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Ewing RM, Chu P, Elisma F, etal . Large-scale mapping of human protein–protein interactions by mass spectrometry . Mol. Syst. Biol. . 3 . 1. 89 . 2007 . 17353931 . 10.1038/msb4100134 . 1847948 .

Notes and References

1. Das BK . Xia L . Palandjian L . Gozani O . Chyung Y . Reed R . Characterization of a Protein Complex Containing Spliceosomal Proteins SAPs 49, 130, 145, and 155 . Mol Cell Biol . 19 . 10 . 6796–802 . February 2000 . 10490618 . 84676 . 10.1128/mcb.19.10.6796.
2. Web site: Entrez Gene: SF3B3 splicing factor 3b, subunit 3, 130kDa.
3. Will . Cindy L . Urlaub Henning . Achsel Tilmann . Gentzel Marc . Wilm Matthias . Lührmann Reinhard . September 2002 . Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein . EMBO J. . 21 . 18 . 4978–88 . 0261-4189. 12234937 . 10.1093/emboj/cdf480 . 126279 .
4. Martinez . E . Palhan V B . Tjernberg A . Lymar E S . Gamper A M . Kundu T K . Chait B T . Roeder R G . October 2001 . Human STAGA Complex Is a Chromatin-Acetylating Transcription Coactivator That Interacts with Pre-mRNA Splicing and DNA Damage-Binding Factors In Vivo . Mol. Cell. Biol. . 21 . 20 . 6782–95 . 0270-7306. 11564863 . 10.1128/MCB.21.20.6782-6795.2001 . 99856 .