SF3B2 explained

Splicing factor 3B subunit 2 is a protein that in humans is encoded by the SF3B2 gene.^{[1] [2]}

Function

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.

Interactions

SF3B2 has been shown to interact with SF3B4,^{[3] [4]} RBM7,^[5] SF3B1^{[6] [7]} and CDC5L.^[8]

Further reading

• Agell N, Aligué R, Alemany V, Castro A, Jaime M, Pujol MJ, Rius E, Serratosa J, Taulés M, Bachs O . New nuclear functions for calmodulin . Cell Calcium . 23 . 2–3 . 115–21 . 1998 . 9601606 . 10.1016/S0143-4160(98)90109-9 .
• Champion-Arnaud P, Reed R . The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site . Genes & Development . 8 . 16 . 1974–83 . Aug 1994 . 7958871 . 10.1101/gad.8.16.1974 . free .
• Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, Sleeman J, Lamond A, Mann M . Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex . Nature Genetics . 20 . 1 . 46–50 . Sep 1998 . 9731529 . 10.1038/1700 . 585778 .
• Das BK, Xia L, Palandjian L, Gozani O, Chyung Y, Reed R . Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155 . Molecular and Cellular Biology . 19 . 10 . 6796–802 . Oct 1999 . 10490618 . 84676 . 10.1128/mcb.19.10.6796.
• Das R, Zhou Z, Reed R . Functional association of U2 snRNP with the ATP-independent spliceosomal complex E . Molecular Cell . 5 . 5 . 779–87 . May 2000 . 10882114 . 10.1016/S1097-2765(00)80318-4 . free .
• Bryant HE, Wadd SE, Lamond AI, Silverstein SJ, Clements JB . Herpes simplex virus IE63 (ICP27) protein interacts with spliceosome-associated protein 145 and inhibits splicing prior to the first catalytic step . Journal of Virology . 75 . 9 . 4376–85 . May 2001 . 11287586 . 114182 . 10.1128/JVI.75.9.4376-4385.2001 .
• Brand M, Moggs JG, Oulad-Abdelghani M, Lejeune F, Dilworth FJ, Stevenin J, Almouzni G, Tora L . UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation . The EMBO Journal . 20 . 12 . 3187–96 . Jun 2001 . 11406595 . 150203 . 10.1093/emboj/20.12.3187 .
• Will CL, Schneider C, MacMillan AM, Katopodis NF, Neubauer G, Wilm M, Lührmann R, Query CC . A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site . The EMBO Journal . 20 . 16 . 4536–46 . Aug 2001 . 11500380 . 125580 . 10.1093/emboj/20.16.4536 .
• Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ . Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis . RNA . 8 . 4 . 426–39 . Apr 2002 . 11991638 . 1370266 . 10.1017/S1355838202021088 .
• Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R . Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein . The EMBO Journal . 21 . 18 . 4978–88 . Sep 2002 . 12234937 . 126279 . 10.1093/emboj/cdf480 .
• Warner DR, Roberts EA, Greene RM, Pisano MM . Identification of novel Smad binding proteins . Biochemical and Biophysical Research Communications . 312 . 4 . 1185–90 . Dec 2003 . 14651998 . 10.1016/j.bbrc.2003.11.049 .
• Will CL, Schneider C, Hossbach M, Urlaub H, Rauhut R, Elbashir S, Tuschl T, Lührmann R . The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome . RNA . 10 . 6 . 929–41 . Jun 2004 . 15146077 . 1370585 . 10.1261/rna.7320604 .
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP . Large-scale characterization of HeLa cell nuclear phosphoproteins . Proceedings of the National Academy of Sciences of the United States of America . 101 . 33 . 12130–5 . Aug 2004 . 15302935 . 514446 . 10.1073/pnas.0404720101 . 2004PNAS..10112130B . free .
• Lin KT, Lu RM, Tarn WY . The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo . Molecular and Cellular Biology . 24 . 20 . 9176–85 . Oct 2004 . 15456888 . 517884 . 10.1128/MCB.24.20.9176-9185.2004 .
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M . Nucleolar proteome dynamics . Nature . 433 . 7021 . 77–83 . Jan 2005 . 15635413 . 10.1038/nature03207 . 2005Natur.433...77A . 4344740 .
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . Oct 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
• Ardon O, Zimmerman ES, Andersen JL, DeHart JL, Blackett J, Planelles V . Induction of G2 arrest and binding to cyclophilin A are independent phenotypes of human immunodeficiency virus type 1 Vpr . Journal of Virology . 80 . 8 . 3694–700 . Apr 2006 . 16571786 . 1440437 . 10.1128/JVI.80.8.3694-3700.2006 .

Notes and References

1. Gozani O, Feld R, Reed R . Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A . Genes & Development . 10 . 2 . 233–43 . Jan 1996 . 8566756 . 10.1101/gad.10.2.233 . free .
2. Web site: Entrez Gene: SF3B2 splicing factor 3b, subunit 2, 145kDa.
3. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . Oct 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
4. Champion-Arnaud P, Reed R . The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site . Genes & Development . 8 . 16 . 1974–83 . Aug 1994 . 7958871 . 10.1101/gad.8.16.1974 . free .
5. Guo TB, Boros LG, Chan KC, Hikim AP, Hudson AP, Swerdloff RS, Mitchell AP, Salameh WA . Spermatogenetic expression of RNA-binding motif protein 7, a protein that interacts with splicing factors . Journal of Andrology . 24 . 2 . 204–14 . 2003 . 12634307 . 10.1002/j.1939-4640.2003.tb02664.x.
6. Das BK, Xia L, Palandjian L, Gozani O, Chyung Y, Reed R . Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155 . Molecular and Cellular Biology . 19 . 10 . 6796–802 . Oct 1999 . 10490618 . 84676 . 10.1128/mcb.19.10.6796.
7. Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R . Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein . The EMBO Journal . 21 . 18 . 4978–88 . Sep 2002 . 12234937 . 126279 . 10.1093/emboj/cdf480 .
8. Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI . Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry . The EMBO Journal . 19 . 23 . 6569–81 . Dec 2000 . 11101529 . 305846 . 10.1093/emboj/19.23.6569 .