SF3A3 explained

Splicing factor 3A subunit 3 is a protein that in humans is encoded by the SF3A3 gene.^{[1] [2] [3]}

This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20.^[3]

Interactions

SF3A3 has been shown to interact with SF3A1.^[4]

Further reading

• Maruyama K . Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Bonaldo MF . Lennon G . Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Neubauer G, King A, Rappsilber J, etal . Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex . Nat. Genet. . 20 . 1 . 46–50 . 1998 . 9731529 . 10.1038/1700 . 585778 .
• Das R . Zhou Z . Reed R . Functional association of U2 snRNP with the ATP-independent spliceosomal complex E . Mol. Cell . 5 . 5 . 779–87 . 2000 . 10882114 . 10.1016/S1097-2765(00)80318-4 . free .
• Will CL, Schneider C, MacMillan AM, etal . A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site . EMBO J. . 20 . 16 . 4536–46 . 2001 . 11500380 . 10.1093/emboj/20.16.4536 . 125580 .
• Nesic D . Krämer A . Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation . Mol. Cell. Biol. . 21 . 19 . 6406–17 . 2001 . 11533230 . 10.1128/MCB.21.19.6406-6417.2001 . 99788 .
• Jurica MS, Licklider LJ, Gygi SR, etal . Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis . RNA . 8 . 4 . 426–39 . 2002 . 11991638 . 10.1017/S1355838202021088 . 1370266 .
• Will CL, Urlaub H, Achsel T, etal . Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein . EMBO J. . 21 . 18 . 4978–88 . 2002 . 12234937 . 10.1093/emboj/cdf480 . 126279 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Dubois T . Zemlickova E . Howell S . Aitken A . Centaurin-alpha 1 associates in vitro and in vivo with nucleolin . Biochem. Biophys. Res. Commun. . 301 . 2 . 502–8 . 2003 . 12565890 . 10.1016/S0006-291X(02)03010-3 .
• Li J, Hawkins IC, Harvey CD, etal . Regulation of alternative splicing by SRrp86 and its interacting proteins . Mol. Cell. Biol. . 23 . 21 . 7437–47 . 2003 . 14559993 . 10.1128/MCB.23.21.7437-7447.2003 . 207616 .
• Nesic D . Tanackovic G . Krämer A . A role for Cajal bodies in the final steps of U2 snRNP biogenesis . J. Cell Sci. . 117 . Pt 19 . 4423–33 . 2005 . 15316075 . 10.1242/jcs.01308 . free .
• Lin KT . Lu RM . Tarn WY . The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo . Mol. Cell. Biol. . 24 . 20 . 9176–85 . 2004 . 15456888 . 10.1128/MCB.24.20.9176-9185.2004 . 517884 .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Rush J, Moritz A, Lee KA, etal . Immunoaffinity profiling of tyrosine phosphorylation in cancer cells . Nat. Biotechnol. . 23 . 1 . 94–101 . 2005 . 15592455 . 10.1038/nbt1046 . 7200157 .
• Andersen JS, Lam YW, Leung AK, etal . Nucleolar proteome dynamics . Nature . 433 . 7021 . 77–83 . 2005 . 15635413 . 10.1038/nature03207 . 2005Natur.433...77A . 4344740 .
• Tanackovic G . Krämer A . Human splicing factor SF3a, but not SF1, is essential for pre-mRNA splicing in vivo . Mol. Biol. Cell . 16 . 3 . 1366–77 . 2005 . 15647371 . 10.1091/mbc.E04-11-1034 . 551499 .

Notes and References

1. Kramer A . Legrain P . Mulhauser F . Groning K . Brosi R . Bilbe G . Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo . Nucleic Acids Res . 22 . 24 . 5223–8 . February 1995 . 7816610 . 332064 . 10.1093/nar/22.24.5223 .
2. Chiara MD . Champion-Arnaud P . Buvoli M . Nadal-Ginard B . Reed R . Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114 . Proc Natl Acad Sci U S A . 91 . 14 . 6403–7 . August 1994 . 8022796 . 44210 . 10.1073/pnas.91.14.6403 . 1994PNAS...91.6403C . free .
3. Web site: Entrez Gene: SF3A3 splicing factor 3a, subunit 3, 60kDa.
4. Nesic . D . Krämer A . October 2001 . Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation . Mol. Cell. Biol. . 21 . 19 . 6406–17 . United States. 0270-7306. 11533230 . 10.1128/MCB.21.19.6406-6417.2001 . 99788 .