SF3A2 explained

Splicing factor 3A subunit 2 is a protein that in humans is encoded by the SF3A2 gene.^{[1] [2] [3]}

Function

This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein.

Interactions

SF3A2 has been shown to interact with DDX46.^[4]

Further reading

• Chiara MD, Champion-Arnaud P, Buvoli M, Nadal-Ginard B, Reed R . Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114 . Proceedings of the National Academy of Sciences of the United States of America . 91 . 14 . 6403–7 . July 1994 . 8022796 . 44210 . 10.1073/pnas.91.14.6403 . 1994PNAS...91.6403C . free .
• Rain JC, Tartakoff AM, Krämer A, Legrain P . Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution . RNA . 2 . 6 . 535–50 . June 1996 . 8718683 . 1369393 .
• Hong W, Bennett M, Xiao Y, Feld Kramer R, Wang C, Reed R . Association of U2 snRNP with the spliceosomal complex E . Nucleic Acids Research . 25 . 2 . 354–61 . January 1997 . 9016565 . 146436 . 10.1093/nar/25.2.354 .
• Moraitou M, Patrinou-Georgoula M, Guialis A . Structural/functional properties of a mammalian multi-component structure containing all major spliceosomal small nuclear ribonucleoprotein particles . The Biochemical Journal . 332 . 1 . 135–44 . May 1998 . 9576861 . 1219461 . 10.1042/bj3320135.
• Yan D, Perriman R, Igel H, Howe KJ, Neville M, Ares M . CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif . Molecular and Cellular Biology . 18 . 9 . 5000–9 . September 1998 . 9710584 . 109085 . 10.1128/mcb.18.9.5000.
• Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, Sleeman J, Lamond A, Mann M . Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex . Nature Genetics . 20 . 1 . 46–50 . September 1998 . 9731529 . 10.1038/1700 . 585778 .
• Krämer A, Grüter P, Gröning K, Kastner B . Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP . The Journal of Cell Biology . 145 . 7 . 1355–68 . June 1999 . 10385517 . 2133165 . 10.1083/jcb.145.7.1355 .
• Das R, Zhou Z, Reed R . Functional association of U2 snRNP with the ATP-independent spliceosomal complex E . Molecular Cell . 5 . 5 . 779–87 . May 2000 . 10882114 . 10.1016/S1097-2765(00)80318-4 . free .
• Will CL, Schneider C, MacMillan AM, Katopodis NF, Neubauer G, Wilm M, Lührmann R, Query CC . A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site . The EMBO Journal . 20 . 16 . 4536–46 . August 2001 . 11500380 . 125580 . 10.1093/emboj/20.16.4536 .
• Nesic D, Krämer A . Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation . Molecular and Cellular Biology . 21 . 19 . 6406–17 . October 2001 . 11533230 . 99788 . 10.1128/MCB.21.19.6406-6417.2001 .
• Dresser DW, Jamin SP, Atkins CJ, Guerrier D . An expressed GNRP-like gene shares a bi-directional promoter with SF3A2 (SAP62) immediately upstream of AMH . Gene . 277 . 1–2 . 163–73 . October 2001 . 11602354 . 10.1016/S0378-1119(01)00690-4 .
• Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ . Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis . RNA . 8 . 4 . 426–39 . April 2002 . 11991638 . 1370266 . 10.1017/S1355838202021088 .
• Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R . Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein . The EMBO Journal . 21 . 18 . 4978–88 . September 2002 . 12234937 . 126279 . 10.1093/emboj/cdf480 .
• Takenaka K, Nakagawa H, Miyamoto S, Miki H . The pre-mRNA-splicing factor SF3a66 functions as a microtubule-binding and -bundling protein . The Biochemical Journal . 382 . Pt 1 . 223–30 . August 2004 . 15142036 . 1133934 . 10.1042/BJ20040521 .
• Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM . Functional proteomics mapping of a human signaling pathway . Genome Research . 14 . 7 . 1324–32 . July 2004 . 15231748 . 442148 . 10.1101/gr.2334104 .
• Nesic D, Tanackovic G, Krämer A . A role for Cajal bodies in the final steps of U2 snRNP biogenesis . Journal of Cell Science . 117 . Pt 19 . 4423–33 . September 2004 . 15316075 . 10.1242/jcs.01308 . free .

Notes and References

1. Bennett M, Reed R . Correspondence between a mammalian spliceosome component and an essential yeast splicing factor . Science . 262 . 5130 . 105–8 . October 1993 . 8211113 . 10.1126/science.8211113 . 1993Sci...262..105B .
2. Dresser DW, Hacker A, Lovell-Badge R, Guerrier D . The genes for a spliceosome protein (SAP62) and the anti-Müllerian hormone (AMH) are contiguous . Human Molecular Genetics . 4 . 9 . 1613–8 . September 1995 . 8541848 . 10.1093/hmg/4.9.1613 .
3. Web site: Entrez Gene: SF3A2 splicing factor 3a, subunit 2, 66kDa.
4. Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R . Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein . The EMBO Journal . 21 . 18 . 4978–88 . September 2002 . 12234937 . 126279 . 10.1093/emboj/cdf480 .