SF3A1 explained

Splicing factor 3 subunit 1 is a protein that in humans is encoded by the SF3A1 gene.^{[1] [2]}

This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.^[2]

Interactions

SF3A1 has been shown to interact with SF3A3^{[3] [4]} and CDC5L.^[5]

Further reading

• Chiara MD, Champion-Arnaud P, Buvoli M, etal . Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114. . Proc. Natl. Acad. Sci. U.S.A. . 91 . 14 . 6403–7 . 1994 . 8022796 . 10.1073/pnas.91.14.6403 . 44210 . 1994PNAS...91.6403C . free .
• Rain JC . Tartakoff AM . Krämer A . Legrain P . Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution. . RNA . 2 . 6 . 535–50 . 1996 . 8718683 . 1369393 .
• Neubauer G, King A, Rappsilber J, etal . Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex. . Nat. Genet. . 20 . 1 . 46–50 . 1998 . 9731529 . 10.1038/1700 . 585778 .
• Toward a complete human genome sequence. . Genome Res. . 8 . 11 . 1097–108 . 1999 . 9847074 . 10.1101/gr.8.11.1097. free . Sanger Centre . The . Washington University Genome Sequencing Cente . The .
• Krämer A . Grüter P . Gröning K . Kastner B . Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP. . J. Cell Biol. . 145 . 7 . 1355–68 . 1999 . 10385517 . 10.1083/jcb.145.7.1355 . 2133165 .
• Dunham I, Shimizu N, Roe BA, etal . The DNA sequence of human chromosome 22. . Nature . 402 . 6761 . 489–95 . 1999 . 10591208 . 10.1038/990031 . 1999Natur.402..489D . free .
• Das R . Zhou Z . Reed R . Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. . Mol. Cell . 5 . 5 . 779–87 . 2000 . 10882114 . 10.1016/S1097-2765(00)80318-4 . free .
• Gunther M . Laithier M . Brison O . A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening. . Mol. Cell. Biochem. . 210 . 1–2 . 131–42 . 2000 . 10976766 . 10.1023/A:1007177623283 . 1339642 .
• Ajuh P, Kuster B, Panov K, etal . Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry. . EMBO J. . 19 . 23 . 6569–81 . 2001 . 11101529 . 10.1093/emboj/19.23.6569 . 305846 .
• Suzuki Y, Tsunoda T, Sese J, etal . Identification and characterization of the potential promoter regions of 1031 kinds of human genes. . Genome Res. . 11 . 5 . 677–84 . 2001 . 11337467 . 10.1101/gr.gr-1640r. 311086 .
• Will CL, Schneider C, MacMillan AM, etal . A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site. . EMBO J. . 20 . 16 . 4536–46 . 2001 . 11500380 . 10.1093/emboj/20.16.4536 . 125580 .
• Nesic D . Krämer A . Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation. . Mol. Cell. Biol. . 21 . 19 . 6406–17 . 2001 . 11533230 . 10.1128/MCB.21.19.6406-6417.2001 . 99788 .
• Jurica MS, Licklider LJ, Gygi SR, etal . Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. . RNA . 8 . 4 . 426–39 . 2002 . 11991638 . 10.1017/S1355838202021088 . 1370266 .
• Will CL, Urlaub H, Achsel T, etal . Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein. . EMBO J. . 21 . 18 . 4978–88 . 2002 . 12234937 . 10.1093/emboj/cdf480 . 126279 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Beausoleil SA, Jedrychowski M, Schwartz D, etal . Large-scale characterization of HeLa cell nuclear phosphoproteins. . Proc. Natl. Acad. Sci. U.S.A. . 101 . 33 . 12130–5 . 2004 . 15302935 . 10.1073/pnas.0404720101 . 514446 . 2004PNAS..10112130B . free .
• Nesic D . Tanackovic G . Krämer A . A role for Cajal bodies in the final steps of U2 snRNP biogenesis. . J. Cell Sci. . 117 . Pt 19 . 4423–33 . 2005 . 15316075 . 10.1242/jcs.01308 . free .
• Lin KT . Lu RM . Tarn WY . The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo. . Mol. Cell. Biol. . 24 . 20 . 9176–85 . 2004 . 15456888 . 10.1128/MCB.24.20.9176-9185.2004 . 517884 .
• Collins JE, Wright CL, Edwards CA, etal . A genome annotation-driven approach to cloning the human ORFeome. . Genome Biol. . 5 . 10 . R84 . 2005 . 15461802 . 10.1186/gb-2004-5-10-r84 . 545604 . free .

Notes and References

1. Kramer A . Mulhauser F . Wersig C . Groning K . Bilbe G . Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae . RNA . 1 . 3 . 260–72 . January 1996 . 7489498 . 1369079 .
2. Web site: Entrez Gene: SF3A1 splicing factor 3a, subunit 1, 120kDa.
3. Nesic . D . Krämer A . October 2001 . Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation . Mol. Cell. Biol. . 21 . 19 . 6406–17 . United States. 0270-7306. 11533230 . 10.1128/MCB.21.19.6406-6417.2001 . 99788 .
4. Chiara . M D . Champion-Arnaud P . Buvoli M . Nadal-Ginard B . Reed R . July 1994 . Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114 . . 91 . 14 . 6403–7 . UNITED STATES. 0027-8424. 8022796 . 1994PNAS...91.6403C . 10.1073/pnas.91.14.6403 . 44210 . free .
5. Ajuh . P . Kuster B . Panov K . Zomerdijk J C . Mann M . Lamond A I . Dec 2000 . Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry . EMBO J. . 19 . 23 . 6569–81 . ENGLAND. 0261-4189. 11101529 . 10.1093/emboj/19.23.6569 . 305846 .