SENP8 explained

Sentrin-specific protease 8 is an enzyme that in humans is encoded by the SENP8 gene.^{[1] [2] [3]}

NEDD8 (MIM 603171) is a ubiquitin-like protein that becomes conjugated to the cullin (see CUL1; MIM 603134) subunit of several ubiquitin ligases. This conjugation, called neddylation, is required for optimal ubiquitin ligase activity. NEDD8-specific deneddylases, such as NEDP1, or DEN1, are required to process the NEDD8 propeptide at a C-terminal diglycine motif and to remove NEDD8 from cullins (Gan-Erdene et al., 2003).[supplied by OMIM]

Further reading

• Mikolajczyk J, Drag M, Békés M, Cao JT, Ronai Z, Salvesen GS . Small ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs.. J. Biol. Chem. . 282 . 36 . 26217–24 . 2007 . 17591783 . 10.1074/jbc.M702444200 . free .
• Drag M, Mikolajczyk J, Krishnakumar IM, Huang Z, Salvesen GS . Activity profiling of human deSUMOylating enzymes (SENPs) with synthetic substrates suggests an unexpected specificity of two newly characterized members of the family. . Biochem. J. . 409 . 2 . 461–9 . 2008 . 17916063 . 10.1042/BJ20070940 . 12234952 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Wu K, Yamoah K, Dolios G, etal . DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1. . J. Biol. Chem. . 278 . 31 . 28882–91 . 2003 . 12759363 . 10.1074/jbc.M302888200 . free .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs. . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Xirodimas DP, Saville MK, Bourdon JC, etal . Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional activity. . Cell . 118 . 1 . 83–97 . 2004 . 15242646 . 10.1016/j.cell.2004.06.016 . free .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Reverter D, Wu K, Erdene TG, etal . Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1. . J. Mol. Biol. . 345 . 1 . 141–51 . 2005 . 15567417 . 10.1016/j.jmb.2004.10.022 .
• Shen LN, Liu H, Dong C, etal . Structural basis of NEDD8 ubiquitin discrimination by the deNEDDylating enzyme NEDP1. . EMBO J. . 24 . 7 . 1341–51 . 2005 . 15775960 . 10.1038/sj.emboj.7600628 . 1142549 .

Notes and References

1. Mendoza HM, Shen LN, Botting C, Lewis A, Chen J, Ink B, Hay RT . NEDP1, a highly conserved cysteine protease that deNEDDylates Cullins . J Biol Chem . 278 . 28 . 25637–43 . Jul 2003 . 12730221 . 10.1074/jbc.M212948200 . free .
2. Gan-Erdene T, Nagamalleswari K, Yin L, Wu K, Pan ZQ, Wilkinson KD . Identification and characterization of DEN1, a deneddylase of the ULP family . J Biol Chem . 278 . 31 . 28892–900 . Jul 2003 . 12759362 . 10.1074/jbc.M302890200 . free .
3. Web site: Entrez Gene: SENP8 SUMO/sentrin specific peptidase family member 8.