SECISBP2 explained

SECIS-binding protein 2 (commonly referred to as SBP2) is a protein that in humans is encoded by the SECISBP2 gene.^{[1] [2]}

Function

The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. SBP2 is a nuclear protein that functions as a SECIS binding protein, but experimental evidence indicates that SBP2 is cytoplasmic.^[2]

Clinical significance

Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism.

See also

• Thyroid hormone resistance

Further reading

• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Research . 6 . 9 . 791–806 . September 1996 . 8889548 . 10.1101/gr.6.9.791 . free .
• Copeland PR, Fletcher JE, Carlson BA, Hatfield DL, Driscoll DM . A novel RNA binding protein, SBP2, is required for the translation of mammalian selenoprotein mRNAs . The EMBO Journal . 19 . 2 . 306–14 . January 2000 . 10637234 . 305564 . 10.1093/emboj/19.2.306 .
• Hartley JL, Temple GF, Brasch MA . DNA cloning using in vitro site-specific recombination . Genome Research . 10 . 11 . 1788–95 . November 2000 . 11076863 . 310948 . 10.1101/gr.143000 .
• Low SC, Grundner-Culemann E, Harney JW, Berry MJ . SECIS-SBP2 interactions dictate selenocysteine incorporation efficiency and selenoprotein hierarchy . The EMBO Journal . 19 . 24 . 6882–90 . December 2000 . 11118223 . 305907 . 10.1093/emboj/19.24.6882 .
• Lescure A, Allmang C, Yamada K, Carbon P, Krol A . cDNA cloning, expression pattern and RNA binding analysis of human selenocysteine insertion sequence (SECIS) binding protein 2 . Gene . 291 . 1–2 . 279–85 . May 2002 . 12095701 . 10.1016/S0378-1119(02)00629-7 .
• Allmang C, Carbon P, Krol A . The SBP2 and 15.5 kD/Snu13p proteins share the same RNA binding domain: identification of SBP2 amino acids important to SECIS RNA binding . RNA . 8 . 10 . 1308–18 . October 2002 . 12403468 . 1370339 . 10.1017/S1355838202020034 .
• Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A . From ORFeome to biology: a functional genomics pipeline . Genome Research . 14 . 10B . 2136–44 . October 2004 . 15489336 . 528930 . 10.1101/gr.2576704 .
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . October 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
• Dumitrescu AM, Liao XH, Abdullah MS, Lado-Abeal J, Majed FA, Moeller LC, Boran G, Schomburg L, Weiss RE, Refetoff S . Mutations in SECISBP2 result in abnormal thyroid hormone metabolism . Nature Genetics . 37 . 11 . 1247–52 . November 2005 . 16228000 . 10.1038/ng1654 . 35631638 .
• Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S . The LIFEdb database in 2006 . Nucleic Acids Research . 34 . Database issue . D415-8 . January 2006 . 16381901 . 1347501 . 10.1093/nar/gkj139 .
• Papp LV, Lu J, Striebel F, Kennedy D, Holmgren A, Khanna KK . The redox state of SECIS binding protein 2 controls its localization and selenocysteine incorporation function . Molecular and Cellular Biology . 26 . 13 . 4895–910 . July 2006 . 16782878 . 1489162 . 10.1128/MCB.02284-05 .

Notes and References

1. Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A . Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs . Genome Research . 11 . 3 . 422–35 . March 2001 . 11230166 . 311072 . 10.1101/gr.GR1547R .
2. Web site: Entrez Gene: SECISBP2 SECIS binding protein 2.