SAOUHSCs221 explained

SAOUHSCs221 antisense RNA
Symbol:	SAOUHSCs221
Altsymbols:	art200, STnc490
Rfam:	XXXX
Rna Type:	Gene sRNA
Tax Domain:	Bacteria

SAOUHSCs221 is a 108nt bacterial antisense RNA found by RNA-seq analysis in Staphylococcus aureus strain HG003 grown in rich medium,^[1] strain JKD60008 ^[2] and strain NCTC8325.^[3]

SAOUHSCs221 is expressed antisense to the IS200 transposase sequence, at the level of the 5’UTR sequence of the transposase ORF. Construction of this family only identified homologues in Firmicutes. However a small RNA named art200 (antisense regulator of transposase IS200) was also described E. coli and S. enterica (enterobacteria).^[4]

Notes and References

1. Liu W, Rochat T, Toffano-Nioche C, Le Lam TN, Bouloc P, Morvan C. Assessment of Bona Fide sRNAs in Staphylococcus aureus. . Front Microbiol . 2018 . 9 . 228 . 29515534 . 10.3389/fmicb.2018.00228 . 5826253 . free .
2. Howden BP, Beaume M, Harrison PF, Hernandez D, Schrenzel J, Seemann T . etal. Analysis of the small RNA transcriptional response in multidrug-resistant Staphylococcus aureus after antimicrobial exposure. . Antimicrob Agents Chemother . 2013 . 57 . 8 . 3864–74 . 23733475 . 10.1128/AAC.00263-13 . 3719707 .
3. Carroll RK, Weiss A, Broach WH, Wiemels RE, Mogen AB, Rice KC . etal. Genome-wide Annotation, Identification, and Global Transcriptomic Analysis of Regulatory or Small RNA Gene Expression in Staphylococcus aureus. . mBio . 2016 . 7 . 1 . e01990-15 . 26861020 . 10.1128/mBio.01990-15 . 4752604 .
4. Ellis MJ, Trussler RS, Haniford DB. A cis-encoded sRNA, Hfq and mRNA secondary structure act independently to suppress IS200 transposition. . Nucleic Acids Res . 2015 . 43 . 13 . 6511–27 . 26044710 . 10.1093/nar/gkv584 . 4513863 .