The Rupert Subdivision is a railroad line owned by CSX Transportation in the U.S. state of West Virginia. It was formerly part of the CSX Huntington East Division.[1] It became part of the CSX Florence Division on June 20, 2016. The line runs from Rainelle, West Virginia, to Clearco, West Virginia, for a total of . At its west end the track wye's off of the Sewell Valley Subdivision and at its east end the track comes to an end.[2]