| Rudi Mathee | |
| Birth Date: | 25 February 1986 |
| Birth Place: | Port Elizabeth, South Africa |
| Weight: | 1080NaN0 |
| School: | Otto du Plessis High School |
| Position: | Lock |
| Years1: | 2007 |
| Apps1: | 6 |
| Points1: | 5 |
| Years2: | 2008–2009 |
| Apps2: | 26 |
| Points2: | 30 |
| Years3: | 2010 |
| Apps3: | 6 |
| Points3: | 10 |
| Years4: | 2010 |
| Clubs4: | → |
| Apps4: | 6 |
| Points4: | 10 |
| Years5: | 2011–2016 |
| Apps5: | 67 |
| Points5: | 60 |
| Years6: | 2014 |
| Apps6: | 5 |
| Points6: | 0 |
| Repyears1: | 2007 |
| Repteam1: | South Africa Students |
| Repcaps1: | 1 |
| Reppoints1: | 5 |
| Repyears2: | 2009 |
| Repteam2: | Highveld XV |
| Repcaps2: | 1 |
| Reppoints2: | 0 |
| Repyears3: | 2012 |
| Repteam3: | South African Barbarians (North) |
| Repcaps3: | 1 |
| Reppoints3: | 0 |
| Clubupdate: | 9 October 2015 |
| Repupdate: | 31 May 2015 |
Rudi Mathee (born 25 February 1986) is a South African former rugby union footballer that played mainly as a lock. He most recently represented the Pumas in the Currie Cup and Vodacom Cup having previously played for the Golden Lions, Leopards, Cheetahs and Griffons.
He was a member of the Pumas side that won the Vodacom Cup for the first time in 2015, beating 24–7 in the final. Mathee made nine appearances during the season, scoring three tries.
He retired in 2016 on medical advice after suffering a back injury.