Roux culture bottle explained

A Roux culture bottle, or simply Roux bottle, is a type of laboratory glassware used in biology and related sciences to grow microorganisms or tissue cells. It consists of a bottle of transparent glass or plastic with two closely spaced flat, rectangular, parallel faces and a short neck; of such a design that the bottle can be laid down sideways, on one of those two faces, even when unstoppered and partially filled with a culture medium. This goal is achieved by having the neck narrowed, offset, partially blocked, or canted (tilted). The item is also generically called cell culture bottle or tissue culture (TC) bottle, and flask may be used instead of "bottle".

A Roux bottle provides a large surface for the cells or microorganisms to grow, whether on the top of, floating in, or at the bottom of the medium. The flat upper face then allows inspection of the culture and even illumination for photosynthetic organisms. Organisms growing at the bottom of the medium can also be inspected from below, with an inverted microscope.

The bottle is typically closed with a plug or cap that prevents contamination of the culture by spores from the outside, while still allowing exchange of gases generated or consumed by the organisms inside.

The bottle's invention has been attributed to French physician and Pasteur's collaborator Pierre Roux (1853-1933).

Variations

The "DeLong" type has a horizontal neck with a low barrier at the base. There is a cylindrical version that is meant to be slowly tuned on rollers to keep the contents well mixed.

See also

• Petri dish
• Kolle culture flask

References

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Notes and References

1. Erhard F. Kaleta and Catherine P. A. Rülke (2009): "The beginning and spread of Fowl Plague (H7 High Pathogenicity Avian Influenza) across Europe and Asia (1878-1955)". In David E. Swayne (ed), Avian Influenza. pages 145-189.
2. Erhard F. Kaleta (2006): "A Brief History, Modes of Spread and Impact of Fowl Plague Viruses". Asia-Pacific Biotech News, volume 10, issue 14, pages 717-725.
3. Christopher G. Morris, Christopher W. Morris : Academic Press Dictionary of Science and Technology. 2432 pages.
4. Orioner, Malaysia (2020): "Pigeons Type Cell Culture Bottle by Sichuan Shubo". Online catalog item 1481-145. Accessed on 2020-02-21.
5. Sarstedt Co. (2020): "TC Flask T175,Standard". Online catalog item 83.3912. Accessed on 2020-02-21.
6. Chemglass Co. (2020): "BOTTLES, ROUX, 1200ML". Online catalog item CLS-1195-1200. Accessed on 2020-02-21.
7. Kisker Biotech GmbH (2020): "Roux flask 38 mm 1200 mL long neck". Online catalog item 202940. Accessed on 2020-02-21.
8. Sigma-Aldrich (2020): "Pyrex Roux culture flask, off-set neck" Online catalog entry SLW1350/04M. Accessed on 2020-02-21.
9. Carl Roth GmbH (2015): "Cell culture bottles Filter screw cap". Online catalog item CE48.1. Accessed on 2020-02-21.
10. Carl Roth GmbH (2015): "Cell culture bottles CELLMASTER Plastic roller bottles Filter screw cap". Online catalog item CE59.1. Accessed on 2020-02-21.
11. Bellco Glass (2020): "DeLong Roux Culture Bottle 1200mL Schott Blank". Online catalog item, SKU 5630-12000. Accessed on 2020-02-21.
12. Marjory Stephenson and Margaret Dampier Whetham (1922): "Studies in the Fat Metabolism of the Timothy Grass Bacillus". Proceedings of the Royal Society of London, Series B - Biology, volume 93, pages 262-280.
13. Sophie A. Leliévre (2015): "Cell Culture Basics 11: Adding medium to a culture flask with attached cells". Lab technique training video produced by Purdue University, uploaded to YouTube.com on 2015-09-23. Accessed on 2020-02-21.
14. Abnova (2011): "Cell Culture (Attached Cell)". Technical instruction video, uploaded to YouTube on 2011-01-13. Accessed on 2020-02-21.
15. Blaine Carter and Michael Huesemann (2008): "Hydrogen production by the cyanobacterium Plectonema boryanum: Effects of initial nitrate concentration, light intensity, and inhibitionof photosystem II by DCMU". U.S. Department of Energy, Journal of Undergraduate Research, OSTI article 1052083, volume 8, pages 23-32.
16. John W. Eyre (1913): Bacteriological Techniques: A Laboratory Guide. Reprinted as Volume 39 of Historical Science series, Books on Demand, 2010; 556 pages.