| Country: | FRA |
| Type: | N |
| Route: | 427 |
| Length Km: | 40 |
| Direction A: | West |
| Direction B: | East |
| Terminus A: | Route Nationale 60 at Thonnance-lès-Joinville |
| Terminus B: | Route Nationale 65 at Liffol-le-Grand |
| Junction: | Route Départementale 25 at Germay; Route Départementale 19 at Trampot |
The Route Nationale 427 is a French road which linked Thonnance-les-Joinville to Liffol-le-Grand.
This road is 40 km long and crossed the departments of Haute-Marne and Vosges and the regions of Champagne-Ardenne and Lorraine.
In 1972, this road had been downgraded to D427.