| Jurisdiction: | Diocese |
| Santa Rosa de Osos | |
| Latin: | Dioecesis Sanctae Rosae de Osos |
| Rite: | Latin Rite |
| Established: | 5 February 1917 (years ago) |
| Cathedral: | Our Lady of the Rosary of Chiquinquirá Cathedral |
| Area Km2: | 24,037 |
| Population: | 650,200 |
| Population As Of: | 2004 |
| Catholics: | 630,000 |
| Catholics Percent: | 96.9 |
| Bishop: | vacant |
| Map: | Mapa Diocesis de Santa Rosa de Osos.svg |
| Website: | www.diocesissantarosadeosos.org |
The Roman Catholic Diocese of Santa Rosa de Osos (la|Sanctae Rosae de Osos) is a diocese located in the city of Santa Rosa de Osos in the ecclesiastical province of Santa Fe de Antioquia in Colombia.