| Jurisdiction: | Diocese |
| San Francisco de Asís de Jutiapa | |
| Country: | Guatemala |
| Province: | Santiago de Guatemala |
| Date: | 25 January 2016 |
| Denomination: | Catholic Church |
| Sui Iuris Church: | Latin Church |
| Rite: | Roman Rite |
| Bishop: | Antonio Calderón Cruz |
| Metro Archbishop: | Gonzalo de Villa y Vásquez, S.J. |
| Suffragan: | for one --> |
| Suffragans: |
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| Assistant Bishop: | for one --> |
| Archdeacon: | for one--> |
The Diocese of San Francisco de Asís de Jutiapa is a Latin Church ecclesiastical territory or diocese of the Catholic Church in Guatemala. It is a suffragan diocese in the ecclesiastical province of the Archdiocese of Santiago de Guatemala. The diocese's name refers to Francis of Assisi.
Its cathedral is the Catedral San Cristóbal in the episcopal see of Jutiapa, southwestern Guatemala.
Established on 25 January 2016 as the Diocese of San Francisco de Asís de Jutiapa on territory split off from the Diocese of Jalapa.