| Jurisdiction: | Diocese |
| Osório | |
| Latin: | Dioecesis Osoriena |
| Country: | Brazil |
| Rite: | Latin Rite |
| Established: | 10 November 1999 (years ago) |
| Cathedral: | Catedral Nossa Senhora da Conceição |
| Area Km2: | 6,210 |
| Population: | 269,186 |
| Population As Of: | 2004 |
| Catholics: | 226,547 |
| Catholics Percent: | 84.2 |
| Metro Archbishop: | Jaime Spengler |
| Emeritus Bishops: | Thadeu Gomes Canellas |
| Map: | File:Diocese de Osório.svg |
| Website: | www.diocesedeosorio.org |
The Roman Catholic Diocese of Osório (Latin: Dioecesis Osoriena) is a diocese located in the city of Osório in the ecclesiastical province of Porto Alegre in Brazil.