| Jurisdiction: | Diocese |
| Mohale's Hoek | |
| Latin: | Dioecesis Mohaleshoekensis |
| Country: | Lesotho |
| Province: | Maseru |
| Rite: | Roman Rite |
| Sui Iuris Church: | Latin Church |
| Cathedral: | St. Patrick's Cathedral |
| Area Km2: | 5,799 |
| Population: | 533,595 |
| Population As Of: | 2004 |
| Catholics: | 306,248 |
| Catholics Percent: | 57.4 |
| Bishop: | John Joale Tlhomola |
| Emeritus Bishops: | Sebastian Koto Khoarai (1978-2014) |
The Roman Catholic Diocese of Mohale's Hoek (Latin: Mohaleshoeken(sis)) is a diocese located in the city of Mohale’s Hoek in the ecclesiastical province of Maseru in Lesotho.