| Country: | Ukraine |
| Kharkiv-Zaporizhzhia | |
| Jurisdiction: | Diocese |
| Latin: | Dioecesis Kharkiviensis-Zaporizhiensis |
| Local: | Єпархія Харків-Запоріжжя |
| Province: | Lviv |
| Metropolitan: | Lviv |
| Metro Archbishop: | Mieczysław Mokrzycki |
| Auxiliary Bishops: | Jan Sobilo |
| Emeritus Bishops: | Marian Buczek |
| Cathedral: | Кафедральний собор Успіння Пресвятої Діви Марії Cathedral of the Assumption of the Blessed Virgin Mary, Kharkiv |
| Cocathedral: | Прокафедральний собор Бога Отця Милосердного Co-Cathedral of the Merciful Father, Zaporizhzhia |
| Rite: | Latin Rite |
| Area Km2: | 196,300 |
| Population: | 15,621,350 |
| Population As Of: | 2019 |
| Catholics: | 48,430 |
| Catholics Percent: | 0.3 |
| Map: | Kharkiv-Zaporizhia diocese location.png |
| Headquarters: | Kharkiv |
| Coordinates: | 49.9964°N 36.2354°W |
The Diocese of Kharkiv-Zaporizhzhia (Latin: Dioecesis Kharkiviensis-Zaporizhiensis) is a diocese of the Latin Church of the Catholic Church in Ukraine. Pavlo Honcharuk is the current bishop of the diocese. The diocesan seat is the Cathedral of the Assumption of the Blessed Virgin Mary in Kharkiv, and the co-cathedral is the Co-Cathedral of the Merciful Father in Zaporizhzhia.
The diocese was created in 2002, when territory from the dioceses of the Diocese of Kamyanets-Podilskyi, and the Diocese of Kyiv-Zhytomyr was split off and merged. The result created two dioceses with Catholic of around 5 percent, and one diocese with only 0.4, which is one of the lowest for any diocese.
The diocese is a suffragan of the Archdiocese of Lviv of the Latins.