| Jurisdiction: | Diocese |
| Juigalpa | |
| Latin: | Dioecesis Iuigalpensis |
| Country: |  Nicaragua |
| Metropolitan: | Leopoldo José Brenes Solórzano |
| Province: | Province of Managua |
| Area Km2: | 12,435 |
| Population: | 314,000 |
| Population As Of: | 2006 |
| Catholics: | 263,000 |
| Catholics Percent: | 83.8 |
| Parishes: | 16 |
| Denomination: | Roman Catholic |
| Rite: | Roman Rite |
| Established: | 21 July 1962 (years ago) |
| Cathedral: | Cathedral of Our Lady of the Assumption |
| Bishop: | Marcial Humberto Guzmán Saballo |
| Map: | Nicaragua - Diocesi di Juigalpa.png |
The Roman Catholic Diocese of Juigalpa (erected 21 July 1962, as the Territorial Prelature of Juigalpa) is a suffragan of the Archdiocese of Managua. It was elevated on 30 April 1991.